Table 1.
List of 8 candidate genes with proper expression intensity.
Fig 1.
Relative expression of candidate reference genes under high temperature.
Error bars show the standard deviations. All genes were compared with 18S rRNA. The experiment was repeated three times and similar results were obtained.
Fig 2.
CT values of the candidate reference genes in qRT-PCR assay.
Expression data displayed as CT values for each candidate reference gene in all samples. A line across the box is depicted as the median. The boxes indicate the 25th and 75th percentiles. Whiskers represent the maximum and minimum values.
Fig 3.
Expression stability values (M) and ranking of candidate reference genes as calculated by geNorm.
Average expression stability values (M) of reference genes were measured during stepwise exclusion of the least stable reference genes under different conditions. (A) short-term high temperature treatment, (B) long-term high temperature treatment, (C) endosperm development stages.
Fig 4.
Determination of the optimal number of reference genes.
Pairwise variation calculated by geNorm to determine the optimal number of reference genes for accurate normalization under different conditions. (A) short-term high temperature treatment, (B) long-term high temperature treatment, (C) endosperm development stages.
Table 2.
Expression stability values (M) and ranking of candidate reference genes as calculated by NormFinder.