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Fig 1.

Geographic location of albacore sampled.

Circles are proportional to the number of individuals collected. (A) southwest Indian Ocean (n = 42), (B) northwest Indian Ocean (n = 31), (C) South Africa (n = 31), and (D) southeast Atlantic Ocean (n = 32).

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Fig 1 Expand

Table 1.

Summary of the selection steps used to develop microsatellite markers.

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Table 1 Expand

Fig 2.

Read length distribution and number of reads throughout QDD bioinformatics pipeline steps.

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Fig 2 Expand

Table 2.

Microsatellite markers developed for Thunnus alalunga (43) with the corresponding GenBank number.

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Table 2 Expand

Table 3.

Summary statistics of the potentially selective «neutral» microsatellite markers (34) for albacore (Thunnus alalunga).

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Table 3 Expand

Fig 3.

Number of microsatellites detected within good quality reads for primer design purpose and number of microsatellites with a successfully designed primer pair by motif type.

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Fig 3 Expand

Table 4.

Characteristics of two «non-neutral» microsatellite markers for albacore (Thunnus alalunga).

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Fig 4.

Factorial correspondence analysis (FCA) in three dimensions of four albacore populations.

A (grey), B (yellow), C (blue), and D (white) (populations names as defined in Fig 1) with 36 markers (34 «neutral» and 2 «non-neutral» markers).

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Fig 4 Expand

Table 5.

Probability of detecting a particular level of differentiation (FST) among populations of albacore with 1 000 replicates.

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Table 6.

PCR amplification results of 37 microsatellites markers tested on Scombridae species (Thunnus albacares, Thunnus thynnus, Thunnus obesus, Acanthocybium solandri) with 4 or 5 individuals per species.

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