Table 1.
Cytotoxicity and antiviral activity of triterpenoids from fruits of M. azedarach.
Table 2.
Cytotoxicity and antiviral activity of 3-α-tigloyl-melianol and melianone against DENV-2 and WNV.
Fig 1.
Virucidal activity of melianone and 3-α-tigloyl-melianol.
Treatment at 37°C for 45 min. with melianone and 3-tigloyl-melianol at different concentrations doesn’t affect the WNV infectivity. Each value represents a mean of duplicate assays.
Fig 2.
2A. Reduction of WNV titre in presence of not cytotoxic concentrations of melianone (20μM) and 3-tigloyl-melianol (15μM). 2’-C-methylguanosine was used as reference compound (at 4μM). 2B. The BHK-21 monolayers treated with the above compounds did not show significant cytopathic effects at the tested concentrations.
Fig 3.
Melianone, 3-α-tigloyl-melianol and a reference compound were added during the infection and at different times post infection (p.i.). Each value represents a mean of duplicate assays. When added 1 hour or more p.i. the two compounds are not more able to protect BHK-21 cells, differently to reference compound. % of cell viability is reported; value for the untreated infected control is 15%.
Fig 4.
Effect of melianone and 3-α-tigloyl-melianol on WNV adsorption kinetic.
BHK-21 cells were incubated during 0, 15, 30 and 60 min. at 4°C with WNV in the absence or presence of compounds. Each value represents a mean of duplicate assays. Treatment with both compounds doesn’t reduce the virus titre.
Table 3.
Antimycobacterial activity (Minimum Inhibitory Concentration: MIC) of selected limonoids against Mycobacterium tuberculosis.