Fig 1.
Experimental timeline.
Fig 2.
Characterization of [3H]-Dopamine uptake and cAMP accumulation in naïve Wistar and SHR rats.
(A) [3H]-Dopamine uptake after 60 min in the PFC (n = 6–8). (B) Time curve of [3H]-Dopamine uptake (n = 3–6). (C) cAMP levels in PFC slices in basal conditions and after exogenous dopamine stimulation (200 μM) (n = 4–8). Results are expressed as means ± S.E.M. A and B: * = p < 0.05; ** = p < 0.01; *** = p < 0.001, SHR vs. Wistar. C: ** = p < 0.01, Control vs. DA.
Fig 3.
Analysis of SHR [3H]-Dopamine uptake.
[3H]-Dopamine uptake was analyzed 24 h (PN56) and 10 days (PN65) after chronic treatment with MPD, as well as 30 min after a single treatment with MPD in vehicle-treated animals (n = 5–6). Results are expressed as means ± S.E.M. * = p < 0.05; *** = p < 0.001, vs. Control.
Fig 4.
Western Immunoblotting for DAT and D1R.
PFC samples of SHR rats were incubated with anti-DAT (1:500) or D1R (1:500) and anti-Tubulin (1:25000) at PN56 (A and C) or PN65 (B and D) after chronic MPD treatment. Results of densitometry indicated in arbitrary units (n = 3). Results are expressed as means ± S.E.M.
Fig 5.
[3H]-SCH 23390 binding and cAMP accumulation after MPD withdrawal in SHR PFC.
(A) [3H]-SCH 23390 binding in the 24-h (PN56) and 10-day (PN65) MPD withdrawal and control groups (n = 4). (B) Basal cAMP accumulation in the 24-h (PN56) and 10-day (PN65) withdrawal group and after SCH 23390 exposure (50 μM; n = 4–7). Results are expressed as means ± S.E.M. ** = p < 0.01; *** = p < 0.001, vs. Control. a = p < 0.001, vs. SCH. b = p < 0.001, vs. MPD.
Fig 6.
[3H]-Dopamine uptake and cAMP accumulation in MPD-withdrawn SHR and Wistar rats after a challenge with cocaine.
Wistar (A) and SHR (B) [3H]-Dopamine uptake after a challenge with cocaine (10 mg/kg i.p.) in vehicle-treated and MPD-withdrawn rats (n = 5–8). Wistar (C) and SHR (D) cAMP levels in vehicle-treated and MPD-withdrawn rats after a single administration of cocaine (n = 3–6). Results are expressed as means ± S.E.M. * = p < 0.05; ** = p < 0.01; *** = p < 0.001, vs. Control. a = p < 0.01, b = p < 0.001, vs. COC. c = p < 0.01, d = p < 0.001, vs. MPD.