Fig 1.
Cuticular wax phenotype on the flag leaf and spike of four wheat varieties.
Glossy varieties of A14 (A) and Jing 2001 (B), and glaucous varieties of Fanmai 5 (C) and Shanken 99 (D). The scale bars indicate 2 cm.
Fig 2.
Developmental changes in wax component and the total load on the leaf surface.
(A) A14. (B) Jing 2001. (C) Fanmai 5. (D) Shanken 99. Four representative developmental stages (50, 100, 200 and 230 days) were investigated for wax coverage. The absolute amounts of cuticular waxes are expressed as μg dm-2 of leaf blade surface area. Each datum point represents a pooled sampled of at least three wheat leaves. Each value represents the mean of three replicates and error bars indicate SD. The amount of diketones is the sum of β- and OH-β-diketones.
Fig 3.
Chain length changes in the individual wax constituent of leaf surface.
(A) A14. (B) Jing 2001. (C) Fanmai 5. (D) Shanken 99. Each wax constituent is designated by carbon chain length and is labeled by chemical class along the x-axis. Each value represents the mean of three replicates. Error bars = SD.
Fig 4.
Development changes in the spike surface wax component and the total wax load.
(A) A14. (B) Jing 2001. (C) Fanmai 5. (D) Shanken 99. Spike surface wax was measured at six stages (1, 3, 5, 7, 9 and 15 DAH). Wax coverage is expressed as μg g-1 of wheat spikes dry weight. Each datum point represents a pooled sampled of at least three spikes. Each value represents the mean of three replicates and error bars indicate SD. The amount of diketones is the sum of β- and OH-β-diketones.
Fig 5.
Chain length distribution in the individual wax constituent on spikes.
(A) A14. (B) Jing 2001. (C) Fanmai 5. (D) Shanken 99. Each wax constituent is designated by carbon chain length and is labeled by chemical class along the x-axis. Each value represents the mean of three replicates. Error bars = SD.
Fig 6.
Developmental changes of epicuticular wax crystals on glume surfaces.
The six stages of spike development are indicated on the left. The wheat varieties of each column are labeled on the top of the charts. Platelet-shaped wax crystals are indicated by the arrows and the bars indicate 10 μm.