Table 1.
Bacteria used for primer verification and specificity testing of the IMS- real-time PCR.
Fig 1.
Detection of Alicyclobacillus spp. by the proposed real-time PCR assay with the 16S rDNA primer.
(A) The representative real-time PCR amplification curves of various Alicyclobacillus spp. strains; (B) The melting curve analysis for Alicyclobacillus spp. amplicons.
Fig 2.
Sensitivity of the real-time PCR detection of Alicyclobacillus spp..
(A) Real-time PCR amplification of the target gene fragment with different concentratioans. NTC: no template control; (B) Standard curve generated from the data plotted in panel; (C) Melt peaks of real-time PCR products of 10-fold serial dilutions of Alicyclobacillus spp.
Table 2.
Repeatability of Ct values in intra- and inter-assays using the IMS- real-time PCR.
Table 3.
Comparison of the IMS-real-time PCR and the standardized pour plate method in naturally contaminated kiwi fruit juice products detection.