Table 1.
Primer pair information.
Table 2.
Equation parameter.
Table 3.
Comparison of qPCR results with simulation and pollen trap data.
Fig 1.
DNA quantification via qPCR measurements.
DNA concentrations in copies m-3 (cp m-3) are given for the multi-copy ITS region and single-copy BP8 gene for coarse (a) and fine particle filter samples (b) DNA copies m-3 are given for ITS quantification on the primary vertical axis, and on the secondary vertical axis for the BP8 gene. The x-axis shows the time, 2010-03-16 / 2010–0608, during the birch-specific pollination period.
Fig 2.
Comparison between qPCR measurements and COSMO-ART simulation.
(a) DNA concentrations in copies m-3 (cp m-3) of coarse particle filter samples are given for multi-copy ITS region (cp m-3 air: primary vertical axis) and simulations of pollen concentrations from COSMO-ART (sum of pollen grains per m3 air: on secondary vertical axis) during the birch-specific main-pollination period in 2010 (2010-03-30 / 2010-04-27, x-axis) for Mainz (b) Quantification results for single-copy BP8 gene (cp m-3 air: primary y axis) and pollen concentrations from COSMO-ART for Mainz and Löwenstein, as well as pollen concentrations averaged hourly in pollen m-3 sampled via Burkard traps (secondary y axis) are summed corresponding to the sampling interval of the appropriate filter sample(y-axis) during the birch-specific main-pollination period in 2010 (2010-03-30 / 2010-04-27, x-axis). Pollen counts were provided from nearest available pollen measurement station in Löwenstein, Baden-Wurttemberg about 130 kilometers south-east of Mainz.
Table 4.
Statistical Analysis.