Table 1.
Summary of Mass Spectrometry Results.
Fig 1.
Summary of sperm proteomes derived from the three major regions of the mouse epididymis.
(A) Graphical representation of epididymis. Different colors represent the 6 distinct transcriptional segments previously identified [14]. Numbers within the epididymis indicate the number of proteins identified in the caputSP (1536), corpusSP (1720) and caudaSP (1234), respectively. Proteins added (protein gain), or removed (protein loss), from sperm between segments are displayed above and below the epididymis. In total, 732 proteins were added and 1034 proteins were removed (1766 total) from sperm during epididymal transit. (B) Venn diagram illustrating the overlap of protein identification between the three epididymal segments. 765 proteins were found to be common to sperm from all segments. (C) Proportion of proteins gained and lost by segment.
Table 2.
Top 20 proteins in the caputSP, corpusSP and caudaSP by spectral count.
Table 3.
Comparison of top 20 caudaSP proteins (by spectral counts) to the caputSP and corpusSP.
Table 4.
Mouse caput, corpus and cauda sperm proteins with known associated sperm phenotypes.
Table 5.
GO term enrichment in proteins added during epididymal transita.
Fig 2.
Enriched biological process GO categories for proteins added and removed from sperm during epididymal transit.
Network biological process diagram indicating functional enrichment of GO categories for proteins added (green) and removed (red) from sperm during epididymal transit. Color legend at top indicates range of overlap shared by the datasets. The varying shades of color represent nodes and clusters of functional GO categories biased towards proteins added (green) or removed (removed) during transit through the epididymis.
Fig 3.
Region-specific enrichment of proteins unique to each segment and organized by GO categories related to cell location.
Enriched categories are color-coded: caput (red), corpus (green) and corpus (blue) indicating the general overall differences in these protein datasets. The darker the node, the greater enrichment in the indicated categories. White, or gray-colored nodes indicate no specific enrichment between each of the three protein lists.
Fig 4.
Enriched immune system process GO categories for proteins in the caput and cauda sperm proteomes.
Network immune system process diagram depicting GO categories enriched in the caput (blue) and cauda (magenta) sperm proteomes. The darker the node color, the stronger the enrichment while white nodes indicate an equal abundance of that functional category in the list of added and removed proteins.
Table 6.
The β-defensins identified in the mouse sperm proteome.