Fig 1.
Laboratory chow consumed in 12 h food deprived mice at the 1 (A), 2 (B), 4 (C), and 24 (B and D) h measurement intervals after intraperitoneal administration of vehicle or 1.0, 5.0, or 10.0 mg/kg of JWH-015.
Data represent means ± SEM. *P<0.05, significantly different from Vehicle, 1.0 and 5.0 mg/kg JWH-015.
Fig 2.
Laboratory chow consumed in 12 h food deprived mice at the 1 (A), 2 (B), 4 (C), and 24 (B and D) h measurement intervals after intraperitoneal administration of vehicle, 5mg/kg AM630, 10mg/kg JWH-015 or AM630 and JWH-015.
Data represent means ± SEM. *P<0.05, significantly different from Vehicle.
Fig 3.
24 h food intake (A), percentage weight loss (B), and energy efficiency (C) in diet induce obese mice following daily intraperitoneal administration of Vehicle or 10 mg/kg/day of JWH-015 for 21 days.
Data are means of total food intake (A) and percentage weight loss (B) of all animals in each group for each day. Error bars represent the SEM. *P<0.05, significantly different from Vehicle.
Fig 4.
Change in plasma levels of non-esterified fatty acid (NEFA) (A), insulin (B), triglycerides (C), Aspartate Aminotransferase (AST) (D), and glucose tolerance test (GTT) (E) following daily intraperitoneal administration of Vehicle or 10 mg/kg/day JWH-015 for 21 days.
JWH-015 was administered 60 min prior to the injection of a glucose load. Data represent means ± SEM. *P<0.05, significantly different from Vehicle.
Fig 5.
Change in brown adipose tissue (BAT) (A), retroperitoneal white adipose tissue (rWAT) (B), and inguinal white adipose tissue (iWAT) (C) weights, changes in adipocyte cell size in the rWAT (D) and iWAT (G), and distribution of adipocytes in abdominal (E-F) and subcutaneous (H-I) fat pads following daily intraperitoneal administration of Vehicle or 10 mg/kg/day JWH-015, respectively, for 21 days.
Data for brown adipose tissue (BAT) (A), retroperitoneal white adipose tissue (rWAT) (B), inguinal white adipose tissue (iWAT) (C), and cell size in the abdominal and subcutaneous fat pads represent means ± SEM. *P<0.05, significantly different from Vehicle. Scale bar = 200μm.
Fig 6.
Western blot detection of Uncoupling protein 1 (UCP1) (A) protein expression in the brown adipose tissue, and adipose tissue triglyceride lipase (ATGL) (B) interleukin-10 (IL-10) (C), tumor necrosis factor-alpha (TNF-α) (D), and protein kinase A (PKA) RIIβ protein expression in the abdominal white adipose tissue from diet induced obese mice treated with Vehicle or 10 mg/kg/day JWH-015 for 21 days.
Representative western blot from a single animal following the above treatments. Signals from each animal quantified and peptide expression shown as a ratio of housekeeping gene β-Actin (Uncoupling protein 1, adipose tissue triglyceride lipase, and protein kinase A RIIβ) and with β-tubulin (interleukin-10 and tumor necrosis factor-alpha). Results are expressed as mean ± SEM. *P < 0.05, significantly different from Vehicle.
Fig 7.
Forced swim test (A-C) and elevated plus maze (D-E) behavior in mice treated with Vehicle or 10 mg/kg/day JWH-015 for 21 days.
Results are expressed as mean ± SEM. *P < 0.05 significantly different from Vehicle.