Fig 1.
SDS-PAGE of the purified recombinant RhaL1 and PNGase F-treated RhaL1.
Lane 1, PNGase F-treated RhaL1; Lane 2, purified recombinant RhaL1; M, molecular mass markers.
Fig 2.
Effects of temperature (a) and pH (b) on activity (Δ) and stability (■) of recombinant RhaL1.
Data points represent the means ± S.D. of three replicates.
Fig 3.
Effects of reaction conditions on RCC-I synthesis.
(a) initial L-rhamnose concentrations; (b) temperature and reaction time; (c) initial mannitol concentrations. Data points represent the means ± S.D. of three replicates.
Fig 4.
Effects of initial fructose orconcentrations on RCC-II synthesis.
Data points represent the means ± S.D. of three replicates.
Fig 5.
Effects of initial esculin concentrations on RCC-III synthesis.
Data points represent the means ± S.D. of three replicates.
Fig 6.
RCCs syntheses by the recombinant RhaL1 via reverse hydrolysis.
The recombinant enzyme synthesized novel RCCs in the presence of L-rhamnose as donor and mannitol, fructose or esculin as acceptors.