Table 1.
Clinico-pathological data for the two study sets.
Fig 1.
Expression of miR-17, miR-21, miR-145, miR-125, miR-200b, and miR-126 as obtained in the study of the test cases.
(A & B) miR-17 in normal (N) and adenomatous (A) tissue and in adenocarcinoma (AC); (C & D) miR-21 expression in stroma of the adenoma compared to normal tissue and in the cancer-associated stroma cells(*). A small group of positive tumour epithelial cells is also present (white arrow); (E) miR-145 is seen in smooth muscle cells (arrow) and vascular smooth muscle cells (arrowhead); in the magnification (F) a reduced number of miR-145 positive fibroblast-like cells are found in the adenoma (arrow) compared to normal tissue (arrowhead); (G) Faint miR-125b signal was seen in the muscularis mucosa (mm); (H) miR-200b was seen in the epithelial cells at base of the crypts, but in this case also in the epithelial cancer cells; (I) miR-126 is exclusively seen in the endothelial cells; (J) The scramble probe showed only discrete background staining.
Table 2.
Results from the validation study of miR-17, miR-21, and miR-145-expression in the normal-adenoma-adenocarcinoma sequence.
Table 3.
Multivariate mixed effects linear regression of log-miR-17, as obtained by image analysis, in normal, adenomatous and invasive tissue of the colon.
Fig 2.
Expression of miR-17 in colon cancer development.
Increased expression of miR-17 in low grade adenoma (LGA), high grade adenoma (HGA) and adenocarcinoma (AC) of the colon compared to normal tissue
Fig 3.
In situ hybridisation of the miR17-92 cluster members in normal and adenomatous colonic mucosa.
(A) Expression of miR-17; (B) miR-18a; (C) miR-19b; (D) miR-20a; (E) miR-92a and (F) scramble probe in normal (N) and adenomatous (A) tissue. Note the increased staining intensity in the adenomatous crypts compared to the normal crypt.