Table 1.
Antimicrobial activity of bacteriocin recovered from W. confusa cultured in different media against Bacillus cereus ATCC14579.
Fig 1.
Antimicrobial activity and the growth of the bacteria.
The antimicrobial activity started to be detected at 8 hours of incubation period and reached optimum at 18 hours of incubation period.
Table 2.
Inhibition zones and MIC value of the crude and purified bacteriocin against test bacteria strains.
Fig 2.
Antimicrobial activity of different fractions from Amberlite XAD 16 column (a) and two different fractions (< 2kDa and 2–5kDa) from Vivaspin (b).
Fig 3.
RP-HPLC profile of active faction isolated from Weissella confusa with antimicrobial activity detected during 36 to 39 minute elution period.
Fig 4.
Effect of various concentration of bacteriocin on the growth of B. cereus at 37°C.
The optical density was measured at 615 nm every hour continuously for a period of 24 hours.
Table 3.
Heat, pH and Enzyme stability tests of the bacteriocin against Bacillus cereus ATCC14579.
Fig 5.
MALDI-TOF mass spectrometry and SDS-PAGE of purified bacteriocin.
MALDI-TOF MS analysis of the HPLC fraction and SDS-PAGE gel picture. Lane 1 is fraction from HPLC, Lane M is Precision Plus Protein™ Dual Xtra Standards (Bio-Rad, USA).
Fig 6.
Real time PCR fluorescence pattern of bacteriocin from W. confusa, negative control, positive control (NaOH, 1M) and tetracycline.
Fig 7.
Bactericidal effect of Bacteriocin A3 on B. cereus.
TEM images of B. cereus cells before (a) and after (b & c) treatment with bacteriocin extracted from W. confusa. Bar indicates 1μm for (a & c) and 500nm for (b). Arrows indicate destruction of membrane.
Fig 8.
Subsystem feature of the genome sequence of W. confusa A3 analysed with RAST server.
The red box indicated absence of genes in toxins and superantigens and virulence, disease and defense genes.