Table 1.
Primers used to amplify the complete nucleotide sequence of N-GPV isolate SDLC01.
Table 2.
Description of waterfowl parvovirus isolates involved in this study.
Fig 1.
Phylogenetic tree constructed using the neighbor-joining method, based on the sequence of the fragment A of SDLC01 (●) and other waterfowl parvovirus isolates available in GenBank database.
The consensus tree from 1000 bootstrap replicates is shown. The percentage of trees that contained the consensus branch is also shown for each branch. The scale at the bottom shows the number of substitutions inferred per site.
Fig 2.
Detection of viral protein expression in DEF cells infected with N-GPV.
(A) DEF cells infected with SDLC01 (B) negative control was fixed at 24 h post-infection and examined with mouse antiserum against the duck parvovirus.
Fig 3.
Phylogenetic relationship between the SDLC01 (●) and other waterfowl parvovirus isolates available in GenBank database based on the complete genomic sequences in the phylogenetic tree, built using the neighbor-joining method.
Numbers at nodes indicate bootstrap percentages obtained using 1000 replicates.
Fig 4.
Phylogenetic relationships between the SDLC01 isolate (●) in this study and other waterfowl parvovirus isolates in the phylogenetic tree, built using the neighbor-joining method.
The analyses were based on nucleotide sequences of NS (A) and VP1genes (B).
Fig 5.
Phylogenetic relationships between the SDLC01 isolate (●) in this study and other waterfowl parvovirus isolates in the phylogenetic tree, built using the neighbor-joining method.
The analyses were based on amino acid sequences of Rep (A) and VP1proteins (B).
Fig 6.
Sequence alignments of Parvovirus PLA2 Motifs and sPLA2 representatives between SDLC01 strain and Adeno-associated virus -2.