Fig 1.
The Gender Specific Mutation Rate of 10 Commonly Mutated CCRCC Genes.
The percentage of mutation for 10 genes commonly mutated in CCRCC genes were collated for 628 tumors from three large sequencing projects (STAG2 data was only available from two projects) [2–4]. The difference in mutation frequency between genders was assessed by Fisher’s Exact tests. Bold text represents statistically significant differences before Bonferroni correction (p-value < 0.05) and * represents statistically significant differences after Bonferroni correction (p-value < 0.005). The expected percentage of mutations in male or female patients was calculated based on the overall mutation rate and the ratio of males to females. The blue bars represent the male patients with dark blue signifying the TCGA cohort, blue signifying the Sato et al cohort and light blue signifying the Guo et al cohort. The red bars represent the female patients with dark red signifying the TCGA cohort, red signifying the Sato et al cohort and light red signifying the Guo et al cohort.
Table 1.
Analysis of Mutation Frequency in 10 Genes Commonly Mutated in CCRCC.
Fig 2.
Comparative Mutation Frequencies of CCRCC-Associated Genes in Cancer.
The relative mutation frequencies of PBRM1, BAP1 and KDM5C in the three CCRCC cohorts were compared to the large number of cancer genome studies listed within the cBioPortal software (http://www.cbioportal.org/index.do) [6,7]. The three CCRCC cohorts were highlighted in grey with the TCGA cohort referred to as TCGA, Nature 2013, the Sato et al cohort referred to as CGP (Tokyo), Nature Gen. 2013 and the Guo et al cohort referred to as BGI, Nature Gen. 2013.
Fig 3.
Mutation and Gender Specific Survival Analysis.
(A and B) Kaplan-Meier survival analysis graphs were produced comparing samples with or within mutation of either BAP1 or KDM5C for CCRCC TCGA cohort and (C and D) comparing the differences in survival with or without mutation of BAP1 for each gender. Analysis was performed using the cBioPortal software (http://www.cbioportal.org/index.do) [6,7] and mutated samples were represented by red lines and wild-type samples by blue lines. Survival data was available for 421 samples within the CCRCC TCGA cohort consisting of 275 males and 146 females. p-values less than 0.01 were considered statistically significant.