Fig 1.
1 day, 4, 6, 10 weeks postoperative radiographs images for beagles treated with minimally invasive plate osteosynthesis (MIPO) (A-D) and open reduction internal fixation (ORIF) (E-H).
Table 1.
Mean ± SD for outcome measurements (fracture reduction and implant associated parameters for dogs in MIPO and ORIF groups).
The P values indicate significant differences determined by unpaired t-test.
Table 2.
Micro-CT analysis between MIPO and ORIF group at different time points (mean ± SD).
Fig 2.
Micro-CT images of the fracture healing progression in MIPO group (A: 4 weeks; B: 8 weeks; C: 12 weeks) and ORIF group (D: 4 weeks; E: 8 weeks; F: 12 weeks).
Fig 3.
New bone formation and mineralization was monitored histomorphometrically by CA (green), AL (red) and TE (yellow) fluorescent quantification.
The images of (A1-3), (B1-3), (C1-3) and (D1-3) represents the labeling at 2(A1, B1), 4(A2, B2), 6(A3, B3), 8(C1, D1), 9(C2, D2),and 10(C3, D3) weeks after operation. A, C and B, D represents confocal LASER microscope for MIPO group and ORIF group, respectively. A4, B4, C4 and D4 represent merged images of the three fluorochromes for the same group. A5, B5, C5 and D5 represent merged images of the three fluorochromes and a plain confocal laser microscope image. Scale bar = 2mm.
Fig 4.
The graph demonstrates the percentage of each fluorochrome area in two groups (* indicates significant differences p < 0.05).
Fig 5.
The photomicrograph of callus formation and mineralization in fracture area from specimens with Masson staining.
The whole images are representative slices of two groups: 4w (A), 8w (B) and 12w (C) in MIPO Group; 4w (D), 8w (E) and 12w (F) in ORIF Group. Scale bar = 200μm.