Table 1.
list of antibodies used in immunohistochemistry and immunofluorescence.
Fig 1.
Basic histology of human term placenta.
Paraffin-embedded sections of human term placenta are stained with H&E (A), CK7 (B) and E-Cad/VIM/Dapi (C). (D) is a higher magnification of (C). Representative images of at least 3 different placentas are shown.
Fig 2.
Trophoblasts are characterized by keratins expression.
Paraffin-embedded sections of human term placenta are stained with Vimentin to mark the stromal cells in the villi, and with keratin 19 (CK19), keratin 8 (CK8), keratin 5 (CK5) and keratin 7 (CK7) to mark the trophoblast cells. Dapi is used to counterstain the nucleus. Representative images of 3 different placentas are shown.
Fig 3.
Expression pattern of keratin 14.
Paraffin-embedded sections of human term placenta are stained with keratin 14 (CK14) along with Vimentin, keratin 19 (CK19), keratin 8 (CK8) and keratin 7 (CK7). Dapi is used to counterstain the nucleus. Representative images of 3 different placentas are shown. The arrows indicate the presence of CK14.
Fig 4.
Keratins expression in BeWo cells.
Representative immunofluorescent images of BeWo cells stained for the indicated antibodies and for Dapi are shown. The arrow indicates the presence of CK14. These experiments were repeated at least 3 times.
Fig 5.
Keratins expression in primary trophoblast cells.
Representative immunofluorescent images of cytospin preparations of primary trophoblast cells, isolated from human term placenta, stained for the indicated antibodies and for Dapi are shown. The arrows indicate the presence of CK14. These experiments were repeated at least 3 times.
Fig 6.
Expression of keratin 14 in primary trophoblast cells.
Representative immunofluorescent images of cytospin preparations of primary trophoblast cells, isolated from human term placenta, stained for the indicated antibodies and for Dapi are shown. The arrows indicate the presence of CK14. These experiments were repeated at least 3 times.