Fig 1.
Tanytarsus sp.10XL, larva.
Fig 2.
Tanytarsus occultus Brundin, 1949, adult male.
Table 1.
Variable and informative sites, and average nucleotide composition in the aligned COI gene sequences.
Fig 3.
Number of DNA barcodes per morphospecies and barcode clusters based on the neighbor joining tree.
Fig 4.
Histogram of pairwise K2P distances of 2790 aligned sequences.
The figure was a result of analysis with ABGD using the K2P model. The horizontal axis shows the pairwise K2P-distance, and the vertical axis shows the number of pairwise sequence comparisons.
Fig 5.
Neighbor joining subtree of the Tanytarsus lugens species group.
Numbers on branches represent bootstrap support (>70%) based on 500 replicates; scale represents K2P genetic distance.
Fig 6.
Neighbor joining subtree of the Tanytarsus brundini species complex.
Numbers on branches represent bootstrap support (>70%) based on 500 replicates; scale represents K2P genetic distance.
Fig 7.
Neighbor joining subtree of Tanytarsus occultus.
Numbers on branches represent bootstrap support (>70%) based on 500 replicates; scale represents K2P genetic distance.
Fig 8.
Neighbor joining subtree of Tanytarsus sp.3XL from Tibet, China.
Numbers on branches represent bootstrap support (>70%) based on 500 replicates; scale represents K2P genetic distance.
Fig 9.
Neighbor joining subtree of Tanytarsus kiseogi, Tanytarsus oscillans and Tanytarsus unagiseptimus.
Numbers on branches represent bootstrap support (>70%) based on 500 replicates; scale represents K2P genetic distance.
Fig 10.
Neighbor joining subtree of Tanytarsus buckleyi and Tanytarsus glabrescens.
Numbers on branches represent bootstrap support (>70%) based on 500 replicates; the dots indicate the specimens identified morphologically; scale represents K2P genetic distance.
Fig 11.
Neighbor joining subtree of European Tanytarsus lestagei aggregate.
Numbers on branches represent bootstrap support (>70%) based on 500 replicates; scale represents K2P genetic distance.
Fig 12.
Neighbor joining subtree of Asian Tanytarsus lestagei aggregate (Tanytarsus takahashii and Tanytarsus yunosecundus).
Numbers on branches represent bootstrap support (>70%) based on 500 replicates. It should be noted that “Tanytarsus talcahashii” is a misspelling of “Tanytarsus takahashii” in GenBank; scale represents K2P genetic distance.
Fig 13.
The number of DNA clusters according to Objective Clustering at different thresholds.
Fig 14.
The number of the OTUs by the prior intraspecific divergence calculated with ABGD online.
Fig 15.
The red vertical line (left) indicates the single threshold time between inter-intraspecific branching; y axis (left) equals the number of lineages; y axis (center) equals the log likelihood of the single threshold GMYC model; the x axes (left and center) show substitutions per nucleotide site; the red branches (right) on the gene tree indicate estimated delimited species.