Fig 1.
A) Schematic diagram of the Flexi-Chamber: A is the fastening mechanism, B is the neoprene cuff around the base of the coral, C is the urine bag, D is standard silicon tubing used to create a water tight seal with the valve, E is the valve of the urine bag and F is the three-way-valve. B) An example of the Flexi-Chamber set-up.
Fig 2.
Mesh-contour graphs comparing the water acceleration (m-2 s-1) along the X, Y and Z planes inside and outside the Flexi-Chambers.
The graphs show the location of each HOBO in 3D space over the 1 h incubation time. At each logging interval (1 s) an X, Y, Z coordinate was generated and is plotted onto the graph: (A) shows the external water acceleration, and (B) shows the water acceleration within the Flexi-Chamber measured with a Hobo Pendant G data logger.
Fig 3.
The percent light transmission through the Flexi-Chamber and a traditional glass respirometry chamber.
Transmission was tested using a spectroradiometer for PAR and UVB and UVA light. Light was converted from energy (W m-2) to photons (μmol m-2 s-1) (see main text), and percent transmission determined as the amount of light transmitted through the respirometry chamber relative to no chamber present.
Table 1.
A) Oxygen hypoxic conditions (O2Sat) and detectable metabolic change (ΔMet) for internal water volume of the Flexi-Chamber during light conditions for Acropora sp. B) Oxygen anoxic conditions (O2Sat) and detectable metabolic change (ΔMet) for the internal water volume of the Flexi-Chamber during dark conditions for Acropora sp.
Fig 4.
Physiological measurements for S. cf. stellata in Salvador Brazil using a conventional glass chamber and the Flexi-Chamber.
(A) Mean calcification (G) rates and (B) mean photosynthesis and respiration rates for 30 colonies of S. cf. stellata are plotted with standard error.
Fig 5.
Physiological measurements for corals in Morro de São Paulo, Bahia, Brazil.
The ratio of PG to R (panel A), and PG to R ratio plotted against daily G (panel B) for three coral species: P. astreoides, S. cf. stellata and M. harttii for two lagoon study sites. Means are shown with standard error (n = 5).
Table 2.
A) The metabolic (Net photosynthesis (PN), Gross photosynthesis (PG), Respiration (R) and Calcification (G)) activity of three coral species at two sites on the coast of Morro de São Paulo, Bahia, Brazil. Incubations were conducted in March 2015 on five colonies of each species over five replicate days. Data are averages (n = 5) and standard error. Rates are mmol m-2 h-1. B) Model parameters to estimate photosynthesis, respiration and calcification as a function of habitat and coral species with day modelled as a random effect.