Fig 1.
Dense Fine Speckled nuclear pattern on HEp-2000® cells (titer 1:1280).
Indirect Immunofluorescence on Hep2000® cells with a fluorescein-conjugated secondary antibody (Immunoconcept) of the serum of a patient with anti-DFS70 antibodies. Picture was acquired using a LEICA/DM-LB2 microscope (x400 magnification) at 20°C, using camera DFC 300FX and acquisition software IM500 (Leica). Anti-DFS70 antibodies titer was determined by testing successive two-fold dilutions of the serum from 1/80 to 1/1280. Samples were classified as positive if well-defined immunofluorescence patterns were identified at 1/160 dilution.
Table 1.
Description of patients with anti-DFS70 antibodies.
Table 2.
Description of patients with a history of thrombotic and/or obstetric events.
Fig 2.
Hypercoagulable state in thrombotic patients harboring anti-DFS70 antibodies.
APTT ratio of healthy individuals with anti-DFS70 antibodies (DFS-Ab, n = 17), patients with thrombophilia without anti-DFS70 antibodies (n = 46) and patients with thrombosis and anti-DFS70 antibodies (n = 42) are shown. Red horizontal lines represent mean values. Comparisons were made using non parametric Mann-Whitney U-test. P-values under 0.05 were considered significant.