Fig 1.
Distribution of actinomycete genera grown on different media compositions.
Fig 2.
Maximum-likelihood tree of selected actinomycete isolates from the Crete (SBT 686–697) and Milos collection (SBT349-381) as well as their nearest representative strains based on the 16S rRNA gene sequence.
The color legend indicates the genus-level of all sequences. The tree is rooted at Escherichia coli strain KCTC 2441T which serves as outgroup. All bootstrap values >50 (500 resamples) are given in percent at the nodes of the tree.
Table 1.
Anti-trypanosomal activities of sponge associated actinomycetes.
Fig 3.
PCA analysis of 34 extracts from Milos collection.
Scores plot (A) and loadings plot (B) of LC-HRMS data; scores plot (C) and loadings plot (D) of 1H NMR data of 34 extracts. For the scores plot (A and C), the outmost PCA outliers for the HRMS data were colored blue for the antitrypanosomal active extract and red for the inactive extract. Inset shows expansion of the central field of the loadings plot to reveal the dense distribution of resonances between 10.0 and 12.5 ppm.
Fig 4.
Dereplicated metabolites for the outlying mass ion peaks for Streptomyces sp. SBT348.
(A) Compound “hits” for m/z 153.020 [M-H]- and structurally related metabolites from genus Streptomycete, (B) compound “hits” for m/z 152.035 [M-H]-, and (C) m/z 178.015 [M-H]-, and 195.041 [M-H]-. Stereochemistry is shown as predicted from the MarinLit database.
Fig 5.
OPLS-DA analysis of 34 extracts from Milos collection.
Scores plot (A) and S plot (B) of LC-HRMS data; scores plot (C) and S plot (D) of 1H NMR data of 34 extracts. Inset shows expansion of the lower left quadrant representing those of the active group.
Fig 6.
PCA analysis of 12 extracts from Crete collection.
Scores plot (A) and loadings plot (B) of LC-HRMS data; scores plot (C) and loadings plot (D) of 1H NMR data of 12 extracts. For the scores plot (A and C) the outmost PCA outliers for the HRMS data were colored blue while those for the 1H NMR data were colored red.
Fig 7.
Dereplicated metabolites for the outlying mass ion peaks for Micromonospora sp. SBT687.