Fig 1.
A. Timeline of nephrotoxic nephritis experiment. B. Representative Western blot depicting STC1 and GAPDH expression in control kidneys 10 days after the administration of anti-GBM or sham. C & D. Bar graphs represent the mean ± SEM of STC1 protein/GAPDH in control kidneys 10 days after the administration of anti-GBM (n = 3) or sham (n = 4); C, quantitation of both STC1 protein bands relative to GAPDH; D, quantitation of top STC1 protein band relative to GAPDH. E. Representative Western blot depicting STC1 and actin expression in the kidneys of STC1 shRNA Tg and scrambled shRNA Tg mice, 10 days after the administration of anti-GBM antibody. F. Bar graph represents the mean ± SEM of STC1 protein/actin in the kidneys of STC1 shRNA Tg and scrambled shRNA Tg mice, 10 days after the administration of anti-GBM antibody (n = 4).
Fig 2.
Kidney morphology, fibrosis and the expression of STC1 and AQP1 after nephrotoxic nephritis in STC1 knockdown kidneys (STC1 shRNA Tg), compared with control kidneys (scrambled shRNA Tg).
Kidney tissue was harvested 10 days after the administration of anti-GBM antibody and stained with Periodic Acid Schiff’s (PAS), Masson’s trichrome, STC1 and AQP1; 40X-200X magnification. Arrowheads point to casts; G, denotes glomerulus.
Table 1.
Morphometric analysis of nephrotoxic nephritis in STC1 knockdown kidneys vs. controls.
Table shows total number of glomeruli surveyed. Of these glomeruli, the number of glomeruli displaying: cell proliferation; hyalinosis/necrosis; crescent formation; and sclerosis were counted. Also listed, tubulointerstitial (TIN) expansion grade (see methods) and the total number of casts counted in 3 representative kidneys in each group (at 400X magnification).
Fig 3.
Serum creatinine, urine output, urine protein, albuminuria, weight and blood pressure after nephrotoxic nephritis in STC1 knockdown (STC1 shRNA Tg) compared with control mice (scrambled shRNA Tg).
Following nephrotoxic nephritis and compared to baseline, serum creatinine was numerically higher in STC1 knockdown kidneys relative to controls (3-fold vs. 2.5-fold); urine output and proteinuria where numerically lower (2.1-fold vs. 2.85-fold and 12-fold vs. 17-fold, respectively), but the differences between the groups were not statistically significant; albuminuria was increased to a similar degree. Blood pressure and weight were higher following nephrotoxic nephritis in STC1 knockdown kidneys compared to control kidneys.
Fig 4.
Equal binding of sheep IgG to GBM, and deposition of mouse IgG and C3 in STC1 knockdown kidneys and control kidneys.
Ten days after anti-GBM Ab injection, kidney sections were labeled with: rabbit anti-mouse IgG antibodies; rabbit anti-sheep IgG antibodies; or rabbit anti-mouse C3 antibodies. Equal staining for sheep anti-mouse GBM antibody and deposition of mouse IgG and C3 in both STC1 knockdown and control kidneys is illustrated; representative images are shown; 100X-200X magnification. Arrowheads point to glomeruli; lower panels show negative staining in wild type kidneys (control).
Fig 5.
Equal deposition of mouse IgG and IgG isotypes after nephrotoxic nephritis in STC1 knockdown and control kidneys.
Bar graphs depict eluted mouse IgG (A) and IgG isotypes (B & C) from STC1 knockdown and control kidneys after nephrotoxic nephritis. Data represent the means ± SEM of 7 independent determinations for STC1 shRNA Tg and 5 independent determinations for scrambled shRNA Tg. Equal mouse antibody response to sheep IgG after nephrotoxic nephritis in STC1 knockdown and control mice. Bar graphs depict mouse serum IgG isotypes (D & E) after nephrotoxic nephritis in STC1 knockdown and control mice. Data represent the means ± SEM of 7 independent determinations for STC1 shRNA Tg and 5 independent determinations for scrambled shRNA Tg.
Fig 6.
Diminished infiltration with macrophages and T-cells after nephrotoxic nephritis in STC1 knockdown kidneys, compared with controls.
Top panels show representative images for F4/80+ (macrophages and dendritic cells) and CD3+ (lymphocytes) staining; arrowheads point to positively stained cells; 200X magnification. Bar graphs depict macrophage and T-cell count in the glomeruli and interstitial compartment (see methods); data represent the means and ± SEM of 5–7 independent determinations. *, P<0.05.
Table 2.
Mouse Common Cytokines RT2 ProfilerTM PCR Array (using total RNA representing whole kidney) was used to survey the expression of 84 important cytokine genes and growth factors after nephrotoxic nephritis in STC1 knockdown kidneys and controls. Middle column shows fold decrease in gene expression in STC1 knockdown kidneys relative to controls. P values highlighted in bold correspond to genes expressed at significantly lower levels after nephrotoxic nephritis in STC1 knockdown kidneys relative to controls.
Fig 7.
Differential expression of cytokines/chemokines/growth factors by proximal tubule cells in response to H2O2 stress.
Immortalized mouse proximal tubules cells (TKPTS) were treated with vehicle or H2O2; mRNA for select cytokines/chemokines (CCL17, CCL19, CX3CL1 and Mif) and growth factors (Gpi1, Spp1 and VEGF) were quantitated using RT-PCR (see methods). Data are normalized to actin, and represent the mean ± SEM of 6 independent determinations. *, P<0.05; **, P<0.01.