Fig 1.
Morphological observation of Naegleria -like cyst at 100X magnification.
Zone of Escherichia coli clearing progressed and the number of cysts increased markedly.
Fig 2.
Amplicon bands revealed by genus and species specific primers against Naegleria isolates for representative samples.
Genus Naegleria specific amplification (Lane 1–13), Lane 1 Ladder 100bp, Lane 2 Negative control, Lane 3 Positive control, Lane 4–13 Positive samples. N. fowleri specific amplification (14–17), Lane 14. Ladder 100bp, Lane 15 Negative control, Lane 16 Positive control, Lane 17 Positive sample.
Table 1.
Overview of geographic sites sampled for Naegleria spp.
Fig 3.
Multiple sequence alignment for amplicons NGES1-NGES10 using ClustalW program.
Black colour dash (—-) indicates gap, red colour fonts base substitution or base insertion, forward and reverse primers are underlined.
Table 2.
Result of DNA sequencing of the selected amplicons (NGES1-NGES10).
Table 3.
Length and position of base in the ITS-5.8S-ITS2-28S sequence of the selected isolates (NGES1- NGES10).
Fig 4.
Neighbour-joining tree depicting the relationships between test isolates with amplicons (NGES1-NGES10) and reference strains of Naegleria.
Numbers at the notes are percentage-bootstrapping values on 1000 replicates, and only values of > 50% are shown. GenBank accession numbers for reference sequences are indicated at the ends of the species designations.
Fig 5.
Graph depicting the association between temperature of the sampling sites and isolation frequency of Naegleria spp.
Y axis denotes the number of isolation and X axis denotes minimum and maximum temperature range of the sampling sites during the entire season (March to August).