Table 1.
L. infantum Alba-domain protein putative partners identified by co-immunoprecipitation and LC-MS/MS analysis.
Fig 1.
Sequence alignment and phylogeny of Alba-domain proteins in Leishmania, Trypanosoma brucei and T. cruzi species.
(A) Neighbor-joining tree showing the phylogenetic relationship between the Alba-domain proteins of TriTryps. Evolutional distances (scale) were estimated as the number of amino acid substitutions per site, considering Poisson correction. The two subgroups Rpp20-like and Rpp25-like are marked. (B) ClustalW alignment of Rpp20-like Alba-domain proteins merged with the in silico structure prediction of LiAlba1 (LinJ.13.0270) using the Phyre algorithm. The best score was obtained with the Alba protein from Sulfolobus solfataricus (Ss)(NCBI WP_010923153.1). ss: secondary structure in silico prediction; C: coil; H: helix; E: Sheet. Red squares indicate amino acids known to be phosphorylated on these specific genes (T. cruzi and T. brucei). The black star shows the expected position for Sir2 acetylation and the red stars underline the signature motif of the subgroup. Sequence variations in coiled regions between Trypanosoma spp. and Leishmania spp. are underlined with a black bar. LiAlba1 was used for Phyre structure prediction. (C) As in B for the Rpp25-like Alba-domain proteins. Structure prediction of LiAlba3 using Phyre outputs Alba2 from Aeropyrum pernix (Ap) K1 (NCBI WP_010866616.1) as the best match. LiAlba3 (LinJ.34.2410) was used for Phyre structure prediction. LinJ: L. infantum; LmjF: L. major; LtaP: L. tarentolae; LbrM: L. braziliensis; Tb: T. brucei; Tc: T. cruzi.
Fig 2.
Alba-domain proteins co-localize to the cytoplasm of promastigote and amastigote Leishmania life stages.
Direct fluorescence images of recombinant L. infantum promastigotes (Pro) and axenic amastigotes (Ama) at passage 4 co-expressing eYPF-LiAlba1 (green) and mCh-LiAlba3 (red) proteins. Green and red pixels overlapped in the digital images yielding yellow/orange signals. The nucleus (N) and kinetoplastid DNA (K) were stained with DAPI (blue).
Fig 3.
Alba-domain proteins are associated with ribosomal subunits.
Polysome fractionation of L. infantum expressing HA-tagged Alba1 and Alba3 proteins by 15–45% sucrose gradient was carried out using logarithmic phase promastigotes (26°C) (A) or heat-stressed parasites grown O/N at 37°C (B). Graphical representations present the RNA content of each collected fraction after ultracentrifugation on 15–45% sucrose gradient. F: Free RNA; 40S, 60S and 80S: ribosomal subunits and monosomes, respectively. Each fraction was loaded on 12% SDS-PAGE and transferred on a nylon membrane for Western blot analysis to detect HA-LiAlba3 and LiAlba1-HA proteins using an anti-HA antibody. As a control, half of the protein extracts were incubated with EDTA before ultracentrifugation to disrupt association of the polyribosomes with mRNAs.
Fig 4.
Alba-domain proteins translocate from the cytoplasm to the flagellum and the nucleolus upon Leishmania amastigote differentiation.
Subcellular localization of LiAlba1-HA and HA-LiAlba3 proteins in promastigotes (A) and upon amastigote differentiation (8 h in MAA medium pH 5.8 at 37°C) (B) was assessed by indirect immunofluorescence studies using an anti-HA antibody as described in Materials and Methods. DAPI staining (red) allows detection of the nucleus (N) and kinetoplastid DNA (K). C) Immunofluorescence images of wild type L. infantum episomally co-expressing pSP-NEOalphaIR-eYPF-LiAlba1 and pSP-HYGalphaIR-mCh-LiNOP10 grown as promastigotes (Pro), differentiating amastigotes (Diff) and amastigotes (Ama). LiNOP10 was used as a nucleolar (Nu) control.
Fig 5.
Heat stress triggers differential localization of Alba-domain proteins in Leishmania.
Immunofluorescence images for eYPF-LiAlba1 (green) and mCh-LiNOP10 (red) in L. infantum promastigotes co-expressing pSP-NEOalphaIR-eYPF-LiAlba1 and pSP-HYGalphaIR-mCh-LiNOP10 submitted to heat stress (from 25°C to 37°C) or to acidic pH (pH 5.8) O/N. Nu: nucleolus.
Table 2.
List of the most representative components of the flagellar structure and RNA metabolism enriched in the L. infantum flagellum proteome.
Fig 6.
Flagellum purification demonstrates an enrichment of LiAlba3 in the Leishmania flagellum during heat stress.
Phase contrast images of intact L. infantum promastigotes (A) and of purified flagella after sucrose gradient isolation (B) as described in Materials and Methods. (C) Summary of MS/MS identified proteins from four independent experiments of flagellum purification (two from promastigote cell extracts and two from 8 h-differentiating amastigotes). Identified genes were classified according to their gene ontology and to characterized orthologs in Trypanosoma spp. based on GeneDB and TriTrypDB gene annotations. Only genes identified at least twice with a minimum of 2 peptides are shown here (see Tables 2, S3 and S4 for the complete list of the identified L. infantum flagellum proteins). (D) Confirmation of flagellar localization of PFR2C-HA in recombinant L. infantum expressing pSP-alphaIRNEOalphaIR-PFR2C-HA. (E) Western blot analysis and quantification of endogenous LiAlba3 in purified flagellum fractions upon promastigote conditions of growth (Pro) or following 8 h of temperature stress (37°C). After flagellum purification, flagella from promastigotes and heat-stressed parasites were counted on a Malassey (hemocytometer) to load an equivalent number of flagella on the gel. Total proteins of promastigote cells were loaded as a control. Relative quantification was performed using ImageJ blot.