Fig 1.
iPSC-derived melanocytes express normal melanocyte markers and produce melanin.
(a-e) Normal epidermal melanocytes (NEM) and (g-k) iPSC-derived cells (at day 11 of the differentiation protocol) express SOX-10, MITF-M and gp-100. Phase contrast images of normal (f) and iPSC-derived (m) cells (at day 11 of the differentiation protocol) showing typical melanocyte morphology. Image (l) represents gp-100 expression in iPSC-derived cells at day 25 of the differentiation protocol. (n) At day 17 of the differentiation protocol expression of melanocyte specific markers is greatly up-regulated in iPSC-derived cells compared to iPSCs. (o) NEM produce melanin as shown by Fontana-Masson staining. (p) & (q) Brightfield microscopy and Fontana-Masson staining, respectively, of iPSC-derived cells (at day 28 of the differentiation protocol) containing melanin. Scale bar = 50μm. Arrows point to melanin as determined by Fontana-Masson staining.
Fig 2.
iPSC-derived keratinocytes can internalize and intracellularly transport microspheres 0.5μm in diameter.
(a) Normal epidermal keratinocytes (NHK) and iPSC-derived keratinocytes (iPSC-Ker) co-cultured with red fluorescent microspheres 0.5μm in diameter for different time points (0, 2, 4 or 6 hours). (b) Quantitative analysis of microsphere internalization. (c) Parallel fluorescence and phase contrast microscopy showing method used to determine that only internalized beads were counted in the quantitative analysis. Blue (DAPI), Red (internalized 0.5μm microspheres).
Fig 3.
iPSC-derived keratinocytes internalize melanosomes in a PAR-2-dependent manner.
a) Normal epidermal keratinocytes (NHK) and iPSC-derived keratinocytes (iPSC-Ker) internalize freshly isolated melanosomes within 24 hours via a mechanism involving PAR-2.–Melanosomes (Fontana-Masson stain of normal and iPSC-derived keratinocytes after 24 hours in culture without the addition of melanosomes), + Melanosomes (Fontana-Masson stain of normal and iPSC-derived keratinocytes after 24 hours in culture with the addition of melanosomes), + Melanosomes & STI (Fontana-Masson stain of normal and iPSC-derived keratinocytes after 24 hours in culture with the addition of melanosomes and soybean trypsin inhibitor). Scale bar = 100μm. b) Quantification of Fontana-Masson staining in a). * = p<0.05, ** = p<0.01, *** = p<0.001.
Fig 4.
iPSC-derived melanocytes and keratinocytes participate in melanin transfer.
(a-b) normal epidermal melanocytes (NEM) and iPSC-derived keratinocytes (iPSK) in co-culture for 7 and 24 hours. (c-d) iPSC-derived melanocytes (iPSM) and normal epidermal keratinocytes (NHK) in co-culture for 7 and 24 hours. (e-h) iPSC-derived melanocytes and iPSC-derived keratinocytes in co-culture for 7 and 24 hours. Inset (enlarged images showing transferred melanin in the perinuclear region).
Fig 5.
3D skin equivalents produced from iPSC-derived fibroblasts, keratinocytes and melanocytes have normal architecture and are functional.
a) Hematoxylin and eosin, b) Ki67, c) gp-100, d) Fontana-Masson, e) loricrin, f) keratin-1, g) keratin-14. Blue (DAPI), dashed line (basement membrane). Inset c) (enlarged image showing an iPSC-derived melanocyte in the basal layer of the epidermis). Inset d) (enlarged image showing an iPSC-derived melanocyte producing melanin). h & i) Control and forskolin-treated iPSC-derived 3D skin equivalents, respectively, after 14 days at the air-liquid interface. j) Quantification of melanin increase in 3D skin equivalents in response to 40μM forskolin. Scale bar = 100μm (except in enlarged images = 10μm). *** = p<0.001.