Fig 1.
Number-averaged carbon number (C#) vs. NSO:C for each sample and control.
Filled symbols indicate data collected from the VSW treatment and open symbols indicate data collected from the VSWE treatment. Circles, squares and triangles indicate DCM 1, DCM 2, and DCM-PPL extracts, respectively. Each extract of the seawater control is plotted in blue. The seawater controls are clustered together within a 95% confidence ellipse. Water-accommodated fractions (WAF) are plotted in purple symbols. Water-soluble fractions (WSF) are plotted in green symbols. The crude oil control is represented by a black asterisk.
Fig 2.
Carbon number vs. nominal mass plot of the DCM 1 extracts of the water-accommodated fraction (WAF; left) and water-soluble fraction (WSF; right) from the VSW treatment.
Each m/z value with an assigned elemental formula is represented by a dot on the figure. The size of each dot corresponds to relative peak height. Components in parent oil are plotted in grey to serve as reference. Color bar indicates ratio of total heteroatoms to carbon atoms (NSO:C).
Fig 3.
Distribution of hydrophilic (NSO:C ≥ 0.49 and molecular mass < 850 Da; white), moderately hydrophobic (0.1 < NSO:C < 0.49 and molecular mass < 850 Da; grey striped), and hydrophobic (NSO:C ≤ 0.1 or molecular mass > 850 Da; black) compounds in the crude oil control and the three extracts of the water-accommodated fraction (WAF) and the water-soluble fraction (WSF) from the VSW treatment.
Hydrophobicity distribution is calculated as the percentage of total formulas from each hydrophobicity class relative to all formulas in a given sample.
Fig 4.
Number-averaged NSO:C values of crude oil (black) and the DCM 1 extracts of the water-accommodated fraction (WAF; striped) and the water-soluble fraction (WSF; white) from the VSW treatment in the mass ranges: < 300 Da, 300 to 600 Da and > 600 Da.