Fig 1.
Evaluation of previously reported reference genes and new candidate reference genes.
A boxplot of RNA-Seq data showing the expression levels of 37 previously reported primer sets from 28 known reference genes and 7 new candidate reference genes. The expression level of each target was shown. White boxes: Group 1 RNA-Seq data; Grey boxes: Group 2 RNA-Seq data. Primer sets with multiple targets were marked with brackets. Primer sets marked in red were chosen for RT-qPCR validation.
Table 1.
RT-qPCR primer sets used in this study and their target genes.
Table 2.
Evaluation of selected reference genes using RNA-Seq data.
Fig 2.
Geomeans of ranking values of (A) tissue types, (B) stress treatments, and (C) all samples.
Genes are arranged in descending order of stability from left to right. Commonly used reference genes are marked with asterisk (*).
Table 3.
Evaluation of reference genes using RT-qPCR assays.
Table 4.
Ranking of reference genes based on the geomean of stability values.