Fig 1.
The expression patterns showing diurnal and circadian rhythm of the E1 gene.
Plants of Harosoy E1 were grown under LDs (16 h light:8 h dark) or SDs (12 h light:12 h dark) before being shifted constant light (LL) or constant darkness (DD). A: LD-LL; B: SD-LL; C: LD-DD; D: SD-DD. Bars indicate standard error of the mean of three replicates.
Fig 2.
Alignment of e1-ba3 with other known E1 mutations.
The SNP mutation site for e1-as, e1-fs are marked by black boxes; and the 5 bp mutation is marked with a red box. The bipartite nuclear localization sites are marked by black dashed line. The B3-like domain is also indicated with a dashed red line.
Fig 3.
Subcellular distribution of the e1-b3a protein in onion epidermal cells.
The e1-b3a-eGFP (A) and the control of eGFP alone (B) were produced transiently under the control of the CaMV 35S promoter and T-Nos in onion epidermal cells and were observed under a fluorescence microscope. The experiments were performed three times, a typical picture was presented.
Fig 4.
The distribution of flowering time (R1) of an F2 population derived from Yanhuang 3 × Zhonghuang 39.
The flowering time (R1) for parents are indicated by arrows; DAE, days after emergence.
Table 1.
The effect of E1 and E2 allelic variations on flowering time (R1) in an F2 population of Yanhuang 3 × Zhonghuang 39.
Table 2.
Statistical analysis of genetic effects of allelic variations at the E1 and the E2 loci on flowering time (R1) in an F2 population of Yanhuang 3 × Zhonghuang 39.
Fig 5.
The distribution of flowering time (R1) of F2 populations derived from Kariyutaka × Moshidougong 503 at Harbin in 2013 (A), in 2014 (B and C), and at Hailun in 2014 (D).
The flowering time (R1) for parents are indicated by arrows; DAE, days after emergence.
Fig 6.
The distribution of flowering time (R1) of an F2 population derived from Kariyutaka × Suzumaru at Harbin in 2013 (A) and in 2014 (B). The flowering time (R1) for parents are indicated by arrows; DAE, days after emergence.