Table 1.
Total number of genes and quantification of highly up-regulated and down-regulated genes versus HuURNA in MPN with or without filtering.
Fig 1.
Comparison of total gene expression in CD34+ cells among MPNs by Venn diagram after 50% filtration.
A) Among JAK2V617F+ ET, PV and PMF; Among JAK2V617F negative ET/PMF (Mut0) and B) JAK2V617F+ ET and PV; C) JAK2V617F+ ET and PMF; D) JAK2V617F+ PV and PMF. Table represents the genes exclusively expressed in a single MPN disorder.
Fig 2.
Comparison of total gene expression in granulocytes among MPNs by Venn diagram after 50% filtration.
A) Among JAK2V617F+ ET, PV and PMF; Among JAK2V617F negative ET/PMF (Mut0) and B) JAK2V617F+ ET and PV; C) JAK2V617F+ ET and PMF; D) JAK2V617F+ PV and PMF. Table represents the genes exclusively expressed in a single MPN disorder.
Table 2.
Cell differentiation, proliferation and apoptosis related genes expression in CD34+ cells and granulocytes of MPN subjects.
Fig 3.
Hierarchical clustering of highly significant genes expressed in CD34+ cells of MPNs.
Statistical significance (p<0.01) was determined among JAK2V617F positive ET (yellow), PV (red), PMF (blue) and JAK2V617F negative ET/PMF (Mut0, green) by one way ANOVA. The numbers on top and below the color bar represent intensity of contrasts with offsets at 0 and 1. The total gene expression of MPNs is also clustered (above image) representing similarities among examined cells. The gene and array correlations were uncentered. The gene description is provided in Table 3.
Table 3.
The statistically significant genes (p<0.01) among MPNs in CD34+ cells determined by microarray analysis.
Table 4.
The common statistically significant genes in comparative proteomic (granulocytes) and microarray analyses (CD34+ cells).
Fig 4.
Expression of genes linked to mTOR signaling pathway in CD34+ cells of MPN origin.
A) Up-regulated genes vs. HuURNA in ET, PV, PMF and ET/PMF subjects without JAK2V617F mutation (Mut0); B) Unchanged gene expression vs. HuURNA in ET, PV, PMF and Mut0 subjects; C) Down-regulated genes vs. HuURNA in ET, PV, PMF and Mut0 subjects analyzed by microarray. Values are mean ± SEM (n = 4–9). *P < 0.05 compared among MPNs.
Fig 5.
mTOR signaling pathway in CD34+ cells of MPN origin.
(+p) phosphorylation, (-p) dephosphorylation; → stimulation, ┴ inhibition; white boxes represent down-regulated genes, gray boxes unchanged genes, black boxes up-regulated genes vs. HuURNA (corresponding to Fig 4), while blue boxes represent none expressed or sporadically expressed genes in MPNs. GFR—growth factor receptors.