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Table 1.

Details of plant material used in the study.

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Fig 1.

Quality score during sequencing cycles of HiSeqTM 2000.

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Table 2.

Length distribution of clustered sequences.

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Fig 2.

Distribution analysis of major classes of microsatellites in safflower genome.

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Fig 3.

Frequency distribution of SSRs with most and least represented repeat motif in each class.

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Fig 4.

Characterization of di- and tri-nucleotide microsatellites discovered in safflower genome.

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Fig 5.

Reiteration units observed for various classes of SSRs in safflower genome.

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Fig 6.

Functional annotation of microsatellite sequences.

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Fig 7.

Distribution of annotated genomic microsatellites of C. tinctorius L. among the Gene Ontology functional classes: Biological process, Cellular component and Molecular function (Level 2).

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Table 3.

Characteristics of 93 polymorphic SSR markers developed for Carthamus tinctorius L.

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Table 4.

Repeat motif type and their rate of polymorphism observed in the present study.

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Fig 8.

Cross-species transferability of 93 polymorphic safflower microsatellite markers in various species of genus Carthamus.

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Fig 9.

Phylogenetic dendrogram based on 93 polymorphic microsatellite markers, elucidating the genetic diversity and relationships among and between safflower accessions and its wild relatives.

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