Table 1.
The Primers for Real-Time RT PCR.
Fig 1.
In Vitro Cultured Fibroblasts Using Different Methods.
A, 70–80% confluency; B, full confluency; C, serum starvation; D, full confluency with serum starvation. Scale bar: 50 μm.
Fig 2.
The Percentages of Fibroblasts in Different Phases of the Cell Cycle was Assayed by Flow Cytometry.
A, 70–80% confluency; B, full confluency; C, serum starvation; D, full confluency with serum starvation.
Table 2.
The Percentages of Fibroblasts in Different Phases of the Cell Cycle under Different Culture Methods.
Fig 3.
The Relative Expression of Cell Cycle-Related Genes was Assayed by Real-Time RT PCR.
The expression of genes in each group was normalized to the control group. Data were collected from three replicates and are presented as the mean±standard deviation. Bars with different lowercase letters are significantly different (p<0.05).
Fig 4.
In Vitro Cultured Cloned Sheep Embryos.
A, 2- and 8-cell embryos; B, early blastocyst; C, hatching blastocyst; D, early blastocyst stained with Hoechst 33342. Scale bar: 20 μm.
Table 3.
The Developmental Ability of Cloned Embryos Derived from Differentially Cultured Fibroblasts.