Fig 1.
Immunohistochemical staining of CT antigens in normal tissues.
Among 22 normal tissues (see materials and methods), ADAM2, CALR3, SAGE1 and MAGE-A proteins were only detected in the germ cells of the testis. More specifically, SAGE-1 and MAGE-A were found in spermatogonia, while ADAM2 and CALR3 were present in post-meiotic germ cells. Only MAGE-A proteins were detected in plancenta (all pictures magnification x20).
Table 1.
ADAM2, CALR3, SAGE1 and MAGE-A expression in lung cancer.
Fig 2.
Immunohistochemical staining of CT antigens in lung and breast cancer tumors.
Examples of lung and breast cancer tumors positive for MAGE-A and negative for ADAM2, CALR3 and SAGE1 (all pictures magnification x20).
Fig 3.
Immunohistochemical staining of SAGE1 in breast cancer and melanoma cell lines.
SAGE1 was detected in the nucleus of FM6 and MDA-MB-435, while other cells lines were negative (all pictures magnification x20).
Table 2.
Analysis of ADAM2, CALR3, SAGE1 and MAGE-A expression in cohort 1 consistent of 68 breast cancers of different clinical stages.
Table 3.
Analysis of ADAM2, CALR3 and MAGE-A expression in cohort 2 consisting of 121 breast cancers of different receptor status.
Fig 4.
Effect of 5-aza-2’-deoxycytidine-treatent on the expression of CT antigens in lung and breast cancer cell lines.
Expression of MAGE-A1, ADAM2, CALR3 and SAGE1 CT antigen genes in 5-aza-2’-deoxycytidine-treated (5 μM, 48 hours) and untreated lung (A) and breast (B) cancer cell lines was measured using quantitative PCR. Error bars = standard deviation.