Fig 1.
Signs and symptoms caused by M. ulei in H. brasiliensis leaflets.
(A) Leaf deformation and sporulation of the GCL012 isolate 7 d post inoculation in RRIM 600. (B) Leaf showing the formation of stromatic structures of the isolate GCL012 at 24 dpi on RRIM 600. (C) A leaf of the clone FX 3864 showing the formation of chlorotic areas at 10 d post inoculation. (D) Germinating conidia of the GCL012 isolate. The germ tube can be observed (450X).
Fig 2.
Distribution of contig lengths using Trinity de novo assembly for the Hevea brasiliensis transcriptome.
All the contigs assembled are included.
Table 1.
Summary data for sequencing and transcriptome assembly of Hevea brasiliensis. De novo and reference-based strategies.
Fig 3.
Length distribution of contigs obtained by using the TopHat-Cufflinks assembly (for reference) for the Hevea brasiliensis transcriptome.
All contigs assembled were included.
Fig 4.
Species distribution (reference-based strategy).
The results generated by sequence homology of transcripts against the NCBI nr database data.
Fig 5.
Distribution of GO terms from BLAST hits of the transcriptome of Hevea brasiliensis (reference-based assembly).
Selected categories of GO terms are displayed at the top of the hierarchy for the biological process, molecular function and cellular component categories.
Fig 6.
Distribution of GO terms from BLAST hits with the transcriptome of Hevea brasiliensis (reference-based assembly).
The biological process, molecular function and cellular component (ontology level 3) categories are shown.
Table 2.
Main specific metabolic pathways associated with the transcriptome of Hevea brasiliensis identified by KEGG.
Fig 7.
Comparison of transcription profiles between samples.
(A) Heat map with hierarchical clustering of transcripts and samples. Significant differential expression values measured in FPKM with (P 0.01) are shown. The colored bars show the relative expression levels for each transcript (row) and each sample (column). (B) Hierarchical clustering using a Spearman correlation matrix resulting from the comparison of transcript expression values (FPKM normalized by TMM) for each pair of samples.
Fig 8.
Groups with common expression profiles.
Each graph corresponds to a hierarchical clustering of transcripts. X-axis: Clone 1 samples at 0 and 48 hpi, clone 2 at 0 and 48 hpi. Y-axis: centered median of log2 (FPKM). The blue line corresponds to the average expression values per group. Gray lines represent individual transcripts. The number of transcripts per group is shown in the upper left part of each graph.