Fig 1.
Schematic of the 2D Liquid Chromatography Mass Spectrometry (2D-LC-MS) setup used in this study. SCX: Strong cation exchange.
Table 1.
Summary of spectral counts and proteins identified.
Fig 2.
Venn diagram showing shared total proteins, CAZymes, FOLymes, and proteases identified in the three gongylidia samples (labeled S1-S3) analyzed in this study.
Fig 3.
Non-metric multidimensional scaling (NMDS) ordination plot of fungus garden and gongylidia samples.
Gongylidia samples are represented by blue circles, while orange squares represent fungus garden samples.
Fig 4.
Heatmaps are presented that show the relative percent of total spectra that could be mapped to specific CAZymes, FOLymes, and proteases (left) and those enzymes that were found to be enriched in at least one gongylidia sample (right; Fisher’s Exact Test, p < 0.005).
Only enzymes identified as enriched in at least one gongylidia sample compared to all fungus garden samples combined are shown.