Fig 1.
Quorum sensing bioassay of quercetin at different concentration (20, 40 and 80μg /ml) showing inhibition of OHL mediated violacein inhibition.
Fig 2.
Inhibition of violacein production in C.violaceum (CECT5999)by quercetin at different concentration (0–80μg/ml).
Line graph represents the percentage inhibition. Vertical bars represent the mean values of triplicates with standard deviation. Same letters in the column are not significantly different (p< 0.05).
Fig 3.
Quantitative analysis for inhibition of biofilm formation in test strains by quercetin at different concentration (0–40μg/ml).
Data represented were mean of triplicate values with standard deviation. Same letters in the column are not significantly different (p< 0.05).
Fig 4.
Quantitative analysis for inhibition of EPS production in test strains by quercetin at different concentration (0–40μg/ml).
Data represented were mean of triplicate values with standard deviation. Same letters in the column are not significantly different (p< 0.05).
Fig 5.
Inhibition of alginate production in P. aeruginosaby quercetin at different concentration(0–80).
Mean values of triplicate independent experiments and SD are shown.
Table 1.
Effect of quercetin on swimming and swarming motility of test strains.
Table 2.
Synergistic effect of quercetin with antibiotics against food-borne pathogens.
Fig 6.
Homology analysis of LasR and YenR gene using clustal W global alignment.
Intensity bar shows the similarity between the sequences. *Represents the identical nucleotides.
Fig 7.
Docked conformation of signaling molecule into the active site of LasR receptor protein.
H-Bonds are displayed in dashed line. Residues, which are forming hydrophobic interaction were labeled.
Table 3.
Details of docked complex of LasR receptor protein with N-octanoyl-DL-homoserine lactone (OHL).
Table 4.
Interaction of LasR receptor protein with quercetin.
Table 5.
Residues of LasR receptor protein interacting with quercetin and N-octanoyl-DL-homoserine lactone (OHL) through various interactions.
Fig 8.
RMSD profile of simulated complexes.
Black color line representssignaling molecule complex with LasR while red color line represents the complex of active compound with LasR.