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Fig 1.

Flowchart of the colposcopic examination and research p16/Ki-67 and hr-HPV.

ASC-US: atypical squamous cells of undetermined significance; LSIL: low-grade squamous intraepithelial lesion; EC: endocervical curettage.

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Fig 1 Expand

Fig 2.

Examples of positive tests for Ki-67 (red) and p16 (brown).

(A) and (D), 200x magnification; (B) and (C), 400x magnification.

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Fig 2 Expand

Fig 3.

Correlation between cervical cytology and colposcopy and histological findings.

CIN1, 2, 3: cervical intraepithelial neoplasia grade 1, 2 and 3; ASC-US: atypical squamous cells of undetermined significance; LSIL: low-grade squamous intraepithelial lesion. (*) Two cases with inconclusive biopsies (1 ASC-US and 1 LSIL) were excluded.

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Fig 3 Expand

Fig 4.

hr-HPV test according to the colpo-histological diagnosis.

HPV-16: positive cytological samples for subtype HPV-16. HPV-18: positive cytological samples for subtype HPV-18. Twelve Other HPV subtypes: positive cytology samples for one or more subtypes of HPV belonging to group 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68. CIN 1, 2, 3: cervical intraepithelial neoplasia grade 1, 2 and 3.

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Fig 4 Expand

Table 1.

Comparative performance of hr-HPV and p16/Ki-67 for the diagnosis of CIN2/CIN3.

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Table 1 Expand