Fig 1.
Typical section of an infiltrating ductal carcinoma (IDC), grade III, showing high tumor cell content of the sample prior to analysis.
Table 1.
Primers used for direct Sanger-sequencing of EGFR-Exon 20
Fig 2.
Real-time PCR analysis of the EGFR-T790M point mutation. The figure shows a representative sample showing the T790M point mutation detected in a patient sample: the control-curve indicates the amplification of a region of exon 2 of the EGFR gene and is used to assess the total DNA in the sample while the T790M-curve indicates the amplification of the T790M-mutant allele in the sample. The other curves labelled with U indicate unspecific amplification of DNA (not present in further analysis).
Fig 3.
Sanger sequencing of the EGFR point mutation in a positive breast cancer sample (A) compared to wild-type control (B). The peak of the mutated allele (ACG > ATG) is indicated by arrow. The upper panel indicates parts of the amino acid sequence encoded by EGFR exon 20.
Table 2.
Patients´ characteristics: frequencies and percentage (n = 131).
Table 3.
Patients´characteristics: three individual Norwegian breast cancer patients with EGFR T790M mutations.
Fig 4.
Overview: Typical EGFR mutations and polymorphisms identified hitherto in sporadic breast cancer.
LB, ligand binding; Auto-phos., autophosphorylation; *present publication.