Fig 1.
A) Histology of the original patient sarcoma. B) Histology of the mouse grown sarcoma.
Fig 2.
Drug-response of soft tissue sarcoma in nude mice.
A representative image of nude mouse with the subcutaneous sarcoma in (A) the untreated mice; (B) GEM-treated; (C) Pazopanib-treated or (D) Salmonella typhimurium A1-R-treated. Scale bars: 10 mm. (E) Growth curves of the subcutaneous sarcoma’s treated with various drugs as described above. The values are mean relative tumor volume ± S.D. (bars) of five different tumors. ** p < 0.01, compared to no treatment group. (F-G) Representative cross-sections of excised subcutaneous tumors from the control and treatment groups with type of treatment indicated.
Fig 3.
Effect of treatment on histology in nude mice.
Histopathological responses to treatments were defined according to Evans’s grading scheme. (A) Treatment effect of control / no treatment was judged as grade I; (B) GEM treatment as grade I; (C) Pazopanib treatment as grade IIa; (D) S. typhimurium A1-R of a viable-like area as grade I and (E) S. typhimurium A1-R of a necrotic area as grade IV. Scale bars: 200 μm.
Fig 4.
Tumor-targeting of S. typhimurium A1-R.
Distribution of GFP-labeled S. typhimurium A1-R in tumors and organs. Representative images of GFP-labeled S. typhimurium A1-R bacteria culture isolated from the tumor and the normal organs (blood and liver) of the mice treated with S. typhimurium A1-R. GFP-labeled S. typhimurium A1-R were clearly detected only in the tumor. Only a few GFP-labeled S. typhimurium A1-R were detected in the liver and no GFP-labeled S. typhimurium A1-R was detected in blood. Scale bars: 10 mm.