Fig 1.
Compost sample codes and description.
Table 1.
First screening of extracellular enzyme activities from the compost in PIIend and PIII-16 analyzed by HPSEC after 24 h incubation.
Fig 2.
Relative enzyme activities in water extracts (4 h incubation) of various stages of mushroom production.
A: exo-activities (in nmol PNP per ml per min). B: total hydrolysis based on reducing sugar determination (in μg ml-1). BGL = β-glucosidase, CBH = cellobiohydrolase, BXL = β-xylosidase, ABF = α-arabinofuranosidase, MND = β-mannosidase. BWX = birchwood xylan, WAX = wheat arabinoxylan, CMC = carboxymethyl cellulose, LBG = locus bean gum.
Table 2.
Total carbohydrate content, carbohydrate molar composition and xylan degree of substitution of compost samples obtained at PII, PIII-16, after filling, after pinning, after 1st and after 2nd flush.
Fig 3.
High performance size exclusion profiles of wheat arabinoxylan (A), birchwood xylan (B), carboxymethyl cellulose (C) and galactomannan (D), after degradation with enzyme extracts obtained from compost of different stages of mushroom growth (24 h incubation).
Fig 4.
HPAEC elution profile of WAX (A) and birchwood xylan (B) incubated (24h) with a: PIIend, b: PIII-16, c: Filling, d: Pinning, e: 1st flush and f: 2nd flush extracellular enzymes.
Ara = arabinosyl, Xyl = xylosyl, X2 = xylobiose, X3 = xylotriose, X4 = xylotetraose, X5 = xylopentaose, AXOS = oligomers substituted with arabinose. GlcAXOS = oligomers substituted with 4-O-methyl-glucuronic acid.
Fig 5.
HPAEC elution profile of WAX incubated (24h) first with 1st flush (e) and then sequentially with pure GH43 AXH-d3 arabinofuranosidase (e*) [17].
Ara = arabinosyl, Xyl = xylosyl, X2 = xylobiose, X3 = xylotriose, X4 = xylotetraose, X5 = xylopentaose, AXOS = oligomers substituted with arabinose.