Fig 1.
Proposed module of GA biosynthesis, inactivation, and perception in Arabidopsis.
Table 1.
Description of genes involved in cytokinin biosynthesis and inactivation.
Table 2.
Description of genes related to cytokinin perception.
Fig 2.
Growth status of carrots from five different developmental stages.
A: Stage 1, 25-day-old. B: Stage 2, 40-day-old. C: Stage 3, 60-day-old. D: Stage 4, 75-day-old. E: Stage 5, 90-day-old. Black lines in the lower left corner of each plant represent 5 cm in that pixel.
Fig 3.
Characterization of root weight and shoot weight during carrot growth and development.
Error bars represent standard deviation among three independent replicates. Data indicate mean ± SD of three replicates.
Fig 4.
Structural changes in the carrot roots from stage 1 (A), stage 2 (B), stage 3 (C, D, and E), stage 4 (F, G, and H), and stage 5 (I, J, and K).
Epidermis (Ep), parenchymalcell (PC), phellogen (Ph), primary phloem (PP), protoxylem (Px), secondary phloem (SP), vascularcambium (VC), and vessel (Ve) are marked in the Figure. Scale bars in A, B, D, E, G, H, J, and K are 100 μm in length, whereas scale bars in C, F, and I are 1 cm in length.
Fig 5.
Structural changes in the carrot petioles from stage 1 (A), stage 2 (B), stage 3 (C), stage 4 (D), and stage 5 (E).
Collenchyma (C), epidermis (Ep), exodermis (Ex), phloem (P), and xylem (X) are marked in the Figure. Scale bars in A, B, C, D, and E are 100 μm in length.
Fig 6.
Structural changes in the carrot leaves from stage 1 (A), stage 2 (B), stage 3 (C), stage 4 (D), and stage 5 (E).
Epidermis (Ep), palisade tissue (Pt), spongy tissue (St), and vascular tissue (V) are marked in the Figure. Scale bars in A, B, C, D, and E are 100 μm in length.
Fig 7.
Bioactive CK levels in different tissues during carrot growth and development.
Error bars represent standard deviation among three independent replicates. Data indicate mean ± SD of three replicates. Different lowercase letters indicate significant differences at P < 0.05.
Fig 8.
qRT-PCR analysis of genes involved in CK biosynthesis and inactivation among different tissues during carrot growth and development.
Error bars represent standard deviation among three independent replicates. Data indicate mean ± SD of three replicates. Different lowercase letters indicate significant differences at P <0.05.
Fig 9.
qRT-PCR analysis of genes involved in CK perception among different tissues during carrot growth and development.
Error bars represent standard deviation among three independent replicates. Data indicate mean ± SD of three replicates. Different lowercase letters indicate significant differences at P <0.05.