Fig 1.
Histograms of relative nuclear DNA content obtained after flow cytometric analysis of propidium iodide-stained nuclei isolated from (A) M. itinerans var. itinerans (ITC.1571) (2C = 1.217 pg); (B) M. rosea (ITC.1598) (2C = 1.285 pg); (C) M. exotica (ITC.1532) (2C = 1.442 pg); (D) M. beccarii var. beccarii (ITC.1516) (2C = 1.537 pg).
Nuclei isolated from soybean (Glycine max, 2C = 2.5 pg) were included as internal reference standard.
Table 1.
Nuclear DNA content, chromosome number and the number of 45S and 5S rDNA loci.
Fig 2.
Relationship between nuclear 2C DNA content and 2n chromosome number in representatives of the genus Musa studied in the present work (empty circles) and by Bartoš et al. [9] (full circles), r = -0.85.
Fig 3.
Examples of genomic distribution of 45S (red, thick arrows) and 5S (green, thin arrows) rRNA genes as localized on mitotic metaphase plates using FISH.
(A) Musa yunnanensis (ITC.1573); (B) Musa mannii (ITC.1574); (C) Musa rosea x siamensis (ITC.1592); (D) Musa campestris var. sarawakensis (ITC.1517); (E) Musa monticola (ITC.1528); (F) Musa lutea (ITC.1515). Chromosomes were counterstained with DAPI. Bar = 5 μm.
Fig 4.
Dendrogram showing the results of the UPGMA analysis based on SSR markers.
Accessions under study were analyzed together with the reference set of diploid entries as used by Christelová et al. [12]. The main clades and subclades are distinguished by colors. The Australimusa/Callimusa clade in green; Rhodochlamys in yellow; BB genotypes in blue; AA genotypes in red and SS genotypes in pink. Accessions analyzed in this study are shown in black.
Table 2.
Sequence characteristics of ITS1-5.8S-ITS2 regions.