Fig 1.
(a) Cultured at 29°C on the surface of potato dextrose agar (PDA) for 7 days; (b) sporangiophore (100x); (c) sporangia (100x); (d) sporangia (200x).
Fig 2.
Sporangium with respect to the time in the spore suspension (1.5x107/flask) inoculated in 250-ml flasks containing 100 ml potato dextrose broth (PDB), with 1%~4% surplus glucose (a) and 1%~4% surplus peptone (b). G: surplus glucose; P: surplus peptone
Fig 3.
(a)1%~4% surplus glucose and (b)1%~4% surplus peptone.
Dried mattress weight with glucose (GA) or peptose (PA); deproteinized mattress weight with glucose (GB) or peptose (PB) of mycelia mattresses formed with respect to the time cultured in potato dextrose broth with glucose (PDBA) or peptose (PDBB).
Fig 4.
Dried mattress weight (right column) and number of sporangium (left column) of mycelia mattresses formed with respect to the time cultured in potato dextrose broth (PDB), with 2% surplus glucose at three different temperatures of 25 (a,b), 29 (c,d), 37°C (e,f).
Fig 5.
Number of sporangium (a) and dried mattress weight (b: Dried mattress weight; c: deproteinized dried mattress weight) for the wild type and its 16 mutants induced by UV radiation cultured in potato dextrose broth (PDB) with 2% surplus glucose at an incubation temperatures of 29°C for four different time periods.
Fig 6.
Dry mattress weight for mutant no. 6 cultured in a flask or tray with three different inoculation densities (a), and non-deproteinized (A) and deproteinized (B) dried mattress weight (b) for mutant no. 6 cultured in potato dextrose broth (PDB) or PDB with 2% surplus glucose (G) for different time periods. Number of sporangium (c) of mutant no. 6 cultured in PDB or PDB with 2% surplus glucose for different time periods.
Fig 7.
Mutant no. 6 cultured in a flask or tray (a); the appearance of the dried mattress (right column) and deproteinized dried mattress (left column) for mutant no. 6 (b) and wild type (c) cultured in potato dextrose agar (PDA) with 2% surplus glucose at 29°C for 8 days.
Fig 8.
a: Thin layer chromatography (visualized with Elson-Morgan reagent) after 16 hr digestion of trifluoroacetic acid for Mutant F6 cultured in PDB or surplus (from left to right) 1.0 or 4% glucose, 1.0 or 4% peptone (lane 1–5, lane 6: N-acetyl glucosamine, lane 7: chitin, lane 8: chitosan, lane 9: glucosamine); b: Thin layer chromatography (visualized with Naphthoresorcinol/ethanol/sulphuric acid reagent) after 16 hr digestion of trifluoroacetic acid for Mutant No. 6 cultured in PDB or surplus (from left to right) 1.0 or 4% glucose, 1.0 or 4% peptone (lane 1–5, lane 6: galactose, lane 7: chitin, lane 8: chitosan, lane 9: glucose).
Fig 9.
Plots of changes in the wound area versus time for those covered with cotton gauze as the control.
Fig 10.
Changes in the amounts of growth factor (PDGF) (a), transforming growth factor (TGF)-β (b), and vascular endothelial growth factor (VEGF) (c) versus time in the wound area covered with cotton gauze, Sacchachitin, Rhizochitin, or BESCHITIN-W.
Fig 11.
Histological staining with hematoxylin and eosin (H&E) for the wound area covered for 3 days with the control (C), cotton gauze, Sacchachitin (S), Rhizochitin (R), or BESCHITIN (B).
Fig 12.
Detection of matrix metalloproteinase (MMP)-9 (92 KDa) and -2 (72 KDa) by gelatin zymography in extracts from the wound area covered for different time periods with the control, cotton gauze (C), Rhizochitin (R), Sacchachitin (S), BESCHITIN-W (B).
(M, marker; P, Protease standard).