Fig 1.
Clonogenic survival fraction of A549, H1299 and BEAS-2B cells irradiated with 125I seeds and 60Co γ-rays.
A-C: the difference in the survival fraction of A549, H1299 and BEAS-2B cells between 125I seeds CLDR radiation and 60Co γ-ray HDR radiation. * indicates a significant difference (P<0.05) when comparing the two radiation treatments and control treatment, and # indicates a significant difference (P<0.05) when comparing 125I seeds CLDR radiation and 60Co γ-ray HDR radiation of the same doses. D, E: The difference in the survival fraction of the three cell lines receiving the same treatment. * indicates a significant difference (P<0.05). Bars, standard error (SE).
Fig 2.
Cell cycle analysis by flow cytometry.
A: G1 arrest of A549 cells irradiated with the two radiation sources; B, C: G2/M arrest of H1299 and BEAS-2B cells after irradiation with the two radiation sources. * indicates a significant difference (P<0.05) when comparing 2, 4, 6 and 8 Gy irradiation by the two radiation sources and control (0 Gy) treatment, and # indicates a significant difference (P<0.05) when comparing the two radiation treatments of the same dose. Bars, standard error (SE).
Fig 3.
Apoptotic ratio of A549, H1299 and BEAS-2B cells irradiated with 125I seeds CLDR radiation and 60Co γ-ray HDR radiation.
A-C: the difference in the apoptotic ratio of A549, H1299 and BEAS-2B cells receiving 125I seeds CLDR radiation and 60Co γ-ray HDR radiation. * indicates a significant difference (P<0.05) when comparing 2, 4, 6 and 8 Gy irradiation by the two radiation sources and control (0 Gy) treatment, and # indicates a significant difference (P<0.05) when comparing the two radiation treatments of the same doses. D, E: the difference in the apoptotic ratios of the three cell lines irradiated with 125I seeds and 60Co γ-rays. * indicates a significant difference (P<0.05). Bars, standard error (SE).
Fig 4.
The expression of Bcl-2 and BAX proteins in A549, H1299 and BEAS-2B cells after exposure to 125I seeds CLDR radiation and 60Co γ-ray HDR radiation of 4 and 8 Gy.
One representative result of three independent experiments with identical results is shown. Con: control.
Fig 5.
The expression of cleaved-caspase-3 and cleaved-PARP proteins in A549, H1299 and BEAS-2B cells after exposure to 125I seeds CLDR radiation and 60Co γ-ray HDR radiation of 4 and 8 Gy.
One representative result of three independent experiments with identical results is shown.