Fig 1.
Survival of adult H. dujardini after exposure to different doses of gamma radiation.
Error bars represent standard deviations from 3 repeats, each with 20 animals.
Fig 2.
Radiation effects on fertility of the tardigrade H. dujardini.
A) Fertility levels expressed as the average number of eggs laid during 12 days after irradiation. B) Egg viability expressed as the proportion of laid eggs that hatched. Error bars represent standard deviations from 3 repeats, each with 20 animals.
Fig 3.
Hatching sequence and success of H. dujardini eggs irradiated at different doses and at two different stages of egg development.
(A) Irradiation at the early developmental stage. (B) Irradiation at the late developmental stage. Time in days after laying are given at the x-axis. Error bars represent standard deviations from 3 repeats, each with 15 eggs.
Fig 4.
Survival of H. dujardini juveniles hatched from eggs irradiated during early or late stage of embryonic development, and their relative fertility.
(A) Young adult survival after irradiation in the early stage of egg development. (B) Young adult survival after irradiation in the late stage of development. (C) Relative fertility of young adults irradiated in the early and late stages, calculated as mean number of eggs laid (in each replicate sample) divided by the highest number of young adults available per repeat.
Fig 5.
Effects of low temperature at irradiation on adult H. dujardini survival.
The figure shows estimates of mean survival over time after irradiation up to 12 days post-irradiation, for samples irradiated on ice (low temperature) and without ice (20°C), and for controls kept at 20°C. Fig (A) shows data for irradiation with 4500 Gy, and Fig (B) for irradiation with 4000 Gy. BR represents survival before radiation and AR represents survival immediately after irradiation. Each data point represents the mean of three repeats, each with 15 animals.
Fig 6.
Mean relative fertility of adult H. dujardini and hatching success of laid eggs after irradiation on ice (A, B) or at room temperature (C, D).
Fertility data points represent the mean number of eggs laid per individual within each repeat sample. Three repeats were used for each dose. Hatching success represents the proportion of laid eggs that hatched within each repeat sample.