Fig 1.
Photomicrographs of Masson’s trichrome stained left ventricular myocardial sections from control mice (A), type I diabetic mice (B), and diabetic mice treated with candesartan (C) or AMD3465 (D).
Bar graph (E) represents the extent of perivascular and interstitial fibrosis. *** p<0.001 vs control. ## p<0.01 vs diabetes. Scale Bar 100 μm.
Table 1.
Gross morphometry in type 1 diabetic mice.
Fig 2.
Photomicrographs of Masson’s trichrome stained left ventricular myocardial sections from untreated sand rats (A and B), and rats treated with AMD3100 (C and D).
Bar graphs showing the extent of perivascular and interstitial myocardial fibrosis. * p<0.05 vs control. Scale Bar 100 μm.
Fig 3.
Bar graphs showing tail-cuff blood pressure.
*** p<0.001 vs control. ### p<0.01 vs diabetes.
Fig 4.
Bar graphs showing the abundance of GFP positive cells per high power field left ventricular myocardium.
Fig 5.
Representative fluorescence photomicrographs demonstrating GFP+ cells (green) and myofibroblasts (alpha smooth muscle actin+ cells, red) in ventricular sections from (A) control, (B) diabetes (C) diabetes+candesartan and (D) diabetes+AMD3465 treated mice.
Bar graph (E) represents quantitative data. Photomicrographic insert in 5B demonstrates co-localization of alpha-SMA and GFP (arrow). *p<0.05 vs diabetes.