Fig 1.
Phenotypic analysis of Col, j594, j2928 and SALK_045148 plants.
(A) Two-week-old seedlings of Col, j594, j2928 and SALK_045148. (B) Five-week-old seedlings of Col, j594, j2928 and SALK_045148. (C) Photographs of representative leaf series of Col, j594, j2928 and SALK_045148 (the fifth true leaves on the left and the youngest leaves on the right). (D) Siliques of Col, j594, j2928 and SALK_045148. Bars = 1 cm.
Fig 2.
PC phenotype in Col, j594, j2928 and SALK_045148.
(A–D) Mature PC shapes of Col (A), j594 (B), j2928 (C), and SALK_045148 (D). Bars = 50 μm. (E–H) Quantitative analyses of the PC area (E), circularity (F), lobe length (G), and lobe number (H) in Col, j594, j2928 and SALK_045148. Error bars indicate s.e.m.; **, P<0.01 by Student’s test.
Fig 3.
SEM images of PCs at different developmental stages in Col, j594, j2928 and SALK_045148.
The top panels, middle panels and bottom panels showed PCs of Col, j594, j2928 and SALK_045148 in stage I, stage II and stage III respectively. The cells with yellow stroke color represented cell shape in each PC developmental stage of Col, j594, j2928 and SALK_045148. Bars = 50 μm.
Fig 4.
j594 and j2928 are two new alleles of CPR1.
(A) Genetic map of the region carrying CPR1 gene. (B) The gene structures of CPR1 (At4g12560.2). Black boxes, exons; gray boxes, the untranslated regions (UTRs); gray line, intron. Arrows indicate the positions of the mutated nucleotide and the T-DNA insertion locus. (C) Two-week-old seedlings of Col, j594/SALK_045148, and j2928/SALK_045148. Bar = 1 cm. (D) Mature PC shapes of Col, j594/SALK_045148, and j2928/SALK_045148. Bars = 50 μm.
Fig 5.
CPR1 rescues the phenotypes of both cpr1-j594 and cpr1-j2928.
(A) Two-week-old seedlings of wild-type, j594, two CPR1/cpr1-j594 lines, j2928, and two CPR1/cpr1-j2928 lines. Bar = 1 cm. (B) Five-week-old seedlings of wild-type, j594, two CPR1/cpr1-j594 lines, j2928, and two CPR1/cpr1-j2928 lines. Bar = 1 cm. (C–G) Mature PC shapes of Col (C), CPR1/cpr1-j594-1# (D), CPR1/cpr1-j594-2# (E), CPR1/cpr1-j2928–1# (F), and CPR1/cpr1-j2928–2# (G). Bar = 50 μm. (H–K) Quantitative analysis of the PC in the complementation test. PC area (H), circularity (I), lobe length (J), and lobe number (K). Error bars indicate s.e.m.; **, P<0.01 by Student’s test.
Fig 6.
Phenotypic analysis in Col, 35S::CPR1, 35S::CPR1I247V and 35S::CPR1∆FBA transgenic plants.
(A) Two-week-old seedlings of Col, 35S::CPR1, three 35S::CPR1I247V transgenic lines and three 35S::CPR1∆FBA transgenic lines. Bar = 1 cm. (B) Mature PC shape of Col, 35S::CPR1, three 35S::CPR1I247V transgenic lines and three 35S::CPR1∆FBA transgenic lines. Bars = 50 μm. (C–F) Quantitative analysis of the PC in the Col, 35S::CPR1, three 35S::CPR1I247V transgenic lines and three 35S::CPR1∆FBA transgenic lines. PC area (C), lobe length (D), circularity (E), and lobe number (F). Error bars indicate s.e.m.; **, P<0.01 by Student’s test.
Fig 7.
CPR1 is required for both MT and MF ordering.
(A–C) Cortical microtubule organization and alignment in PCs of Col (A), cpr1-j594 (B), and cpr1-j2928 (C). (D–F) Microfilaments in PCs from Col (D), cpr1-j594 (E), and cpr1-j2928 (F). Bars = 20 μm.
Fig 8.
Analysis of MT organization in different development stages of PC.
(A, D, G) MT organization in stage I PCs from Col (A), cpr1-j594 (D), and cpr1-j2928 (G). (B, E, H) MT organization in stage II PCs from Col (B), cpr1-j594 (E), and cpr1-j2928 (H), the arrows indicated the neck region. (C, F, I) MT organization in stage III PCs from Col (C), cpr1-j594 (F), and cpr1-j2928 (I). Bars = 20 μm.
Fig 9.
Analysis of MF cytoskeleton in different development stages of PC.
(A, D, G) MF cytoskeleton in stage I PCs from Col (A), cpr1-j594 (D), and cpr1-j2928 (G). (B, E, H) MF cytoskeleton in stage II PCs from Col (B), cpr1-j594 (E), and cpr1-j2928 (H). (C, F, I) MF cytoskeleton in stage III PCs from Col (C), cpr1-j594 (F), and cpr1-j2928 (I). Bars = 20 μm.
Fig 10.
CPR1 regulates PC morphogenesis dependent on natural plant immune response.
(A–I) Genetic interaction analysis between CPR1 and PAD4, EDS1 in PC morphogenesis. (J–L) Mature PC shape of Col, cpr1-j594, cpr1-j2928 growing at 28°C. Bars = 50 μm.
Fig 11.
Quantitative analysis of the PC in the high temperature test and the genetic interaction between CPR1 and PAD4, EDS1.
PC area (A), lobe length (B), circularity (C), and lobe number (D). Error bars indicate s.e.m.; **, P<0.01 by Student’s test.