Fig 1.
Summary statistics of single nucleotide polymorphisms (SNPs) including indels from female and male Eriocheir sinensis unigenes.
(A) Classification of SNPs. Distribution (%) of each SNP and indel type. (B) Venn diagram of common and unique SNPs and indels of female and male unigenes.
Table 1.
Primary sex determination pathway related genes detected in Eriocheir sinensis transcriptomes.
Fig 2.
GO distributions of differentially expressed genes (DEGs) from female and male Eriocheir sinensis transcriptomes.
(A) Different functional distribution of the DEGs involved with biological processes. (B) Different functional distribution of the DEGs involved with cellular components and molecular functions. (C) Differentially expressed functional processes. The horizontal line indicates the significance threshold (p < 0.05).
Fig 3.
Expression pattern of genes involved in lysosomal pathway.
The pathway is based on a KEGG pathway analysis. The up-regulated genes in male and female are labeled by green and red, respectively, and the purple color represents genes with no expression differences between female and male transcriptomes of Eriocheir sinensis.
Table 2.
List of differentially expressed genes related to metabolism in female and male Eriocheir sinensis transcriptomes.
Table 3.
List of differentially expressed genes related to immunity in female and male Eriocheir sinensis transcriptomes.
Table 4.
List of differentially expressed genes related to reproduction in female and male Eriocheir sinensis transcriptomes.
Fig 4.
qRT-PCR validation of selected differentially expressed genes identified by RNA-seq.
Vertical bars represent the mean ± S.E. (n = 3). Significant differences are indicated with an asterisk at P < 0.05, and two asterisks at P < 0.01.