Fig 1.
Schematic illustration of two-chambered MFC for bioelectricity generation.
Fig 2.
Analysis of A. andensis ANESC-ST resilience to different pH conditions.
(A) Growth curves of A. andensis ANESC-ST in LB broth with varied pH conditions (Initial OD600 ≈ 0.4). (B) Cultures of A. andensis ANESC-ST after 14 hours in LB broth with varied pH conditions (Initial OD600 ≈ 0.4).
Fig 3.
Analysis of A. andensis ANESC-ST resilience to different salt concentrations.
(A) Growth curves of A. andensis ANESC-ST in LB broth with varied NaCl concentrations (Initial OD600 ≈ 0.4). (B) A. andensis ANESC-ST was inoculated and cultured for 14 hours in LB broth with varied NaCl concentrations (Initial OD600 ≈ 0.4).
Fig 4.
Bioelectricity generation by MFCs inoculated with A. andensis ANESC-ST operated under different pH and salt concentrations.
(A) Current density vs. time plot of A. andensis ANESC-ST in LB broth (pH = 9.0, 1% NaCl). (B) Current density vs. time plot of A. andensis ANESC-ST in LB broth with pH 7.0, 8.0, 9.0, 10.0, and 11.0. (C) Current density vs. time plot of A. andensis ANESC-ST in LB broth with 1% and 4% NaCl. Data represents an average of triplicates. (D) Power density curve of the MFC inoculated with A. andensis ANESC-ST in LB broth (pH 9 and 1% NaCl).
Fig 5.
CV analysis of A. andensis ANESC-ST in MFCs.
(A) CV traces of MFCs inoculated with fresh M9 media, A. andensis ANESC-ST and free cell spent media of A. andensis ANESC-ST. The working electrode is carbon felt, counter electrode is Pt wire and the reference electrode is Ag/AgCl. Scan rate is 5 mV/s. Voltammograms were acquired in the presence of 10 mM L-Arabinose. (B) Plot of peak current vs. scan rate of the CV traces. All experiments were performed in triplicates.
Fig 6.
Current density vs. time plots of E. coli DH5α and P. aeruginosa lasIrhlI mutant – based MFCs.
EM stands for extracellular metabolites of A. andensis ANESC-ST (2.5 mg/mL). Arrows indicate introduction of EM. Data represents an average of triplicates.
Fig 7.
Current density vs. time graph of A. andensis ANESC-ST in seawater fed MFCs.
Data represents an average of triplicates.