Fig 1.
Inverted DAPI-stained karyotypes of the mourning gecko (Lepidodactylus lugubris, LLU). 3n = 66.
(A) male A and (B) female A. Homologies between LLU and Hemidactylus platyurus (HPL) revealed by chromosome painting are indicated on male karyotype. Scale bar, 10 μm.
Fig 2.
Example of Gekko japonicus painting probes localization onto chromosomes of Lepidodactylus lugubris.
Painting probes specific to chromosomes GJA1 (green) and GJA2 (red) each paints two triplets of LLU (female B) chromosomes: GJA1—LLU3 and LLU16; GJA2 – LLU7 and LLU13. Scale bar, 10 μm.
Table 1.
Homologous regions between several gekkonid species.
Homology between chromosomes of Lepidodactylus lugubris (LLU), Gekko japonicus (GJA), Hemidactylus turcicus (HTU), and H. platyurus (HPL). Homologies between GJA, HTU and HPL have been identified previously [14].
Fig 3.
Localization of ribosomal DNA clusters in the mourning gecko (Lepidodactylus lugubris).
A partial metaphase of the LLU male A painted with human rDNA probe. Arrows indicate NORs in p-arms of two small but different in size chromosomes and in the distal part of the q-arm of a large chromosome. Scale bar, 10 μm.
Fig 4.
Mapping of ribosomal RNA clusters on Lepidodactylus lugubris.
(A) Co-localization of the probe containing chromosomes GJA7+8+9 (green, paints LLU5, 8, 11, 10prox) and ribosomal probe (red) on LLU8 in female A (indicated by an arrow). (B) Co-localization of a probe containing GJA13 and GJA14 (red, paints LLU14 and LLU15) and ribosomal probe (green) on LLU15 (indicated by an arrow) in female B. (C) Co-localization of a probe containing a mixture of GJA15+16 (red, paints LLU17 and LLU18) and ribosomal probe (green) on LLU18 (indicated by an arrow) in male A. Scale bar, 10 μm.
Fig 5.
AgNOR staining of Lepidodactylus lugubris chromosomes.
(A) LLU female B and (B) LLU male D. There is a polymorphism in NOR activity revealed by Ag-staining: only a single chromosome carries an active NOR in female B, but all three NORs are revealed in male D. Scale bar, 10 μm.
Fig 6.
C-banding of Lepidodactylus lugubris chromosomes.
(A) female B and (B) male A. Pericentromeric and subtelomeric heterochromatic regions are dark-staining. Scale bar, 10 μm.
Fig 7.
A scenario of karyotype evolution within Gekko-Lepidodactylus- Hemidactylus group.
Stars indicate chromosome fissions and circles indicate chromosome fusions. The recurrent chromosome rearrangements are underlined. The tree is based on previously published data [19].