Fig 1.
The expression of Npr2 mRNA in goat follicles and COCs.
A, The relative levels of Npr2 mRNA in goat preantral follicles at different developmental stages. B, The relative levels of Npr2 mRNA in mural granulosa cells and COCs in goat antral follicles. C, The expression of Npr2 mRNA in cumulus cells and oocytes of COCs in goat antral follicles. Npr2: natriuretic peptide receptor 2; COCs: cumulus oocyte complexes; GCs: granulosa cells.
Fig 2.
CNP temporarily inhibited the meiotic resumption of goat oocytes cultured in vitro.
COCs were cultured in medium containing different concentrations (0, 50, 100 and 150 ng/mL) of CNP, and the proportion of oocytes with GV was evaluated after 0, 4, 6, 8 hours of culture, respectively. CNP: C-type natriuretic peptide; COCs: cumulus oocyte complexes; GV: germinal vesicle.
Fig 3.
Effect of estradiol on CNP-induced meiotic arrest and the expression of Npr2.
A, Effect of estradiol on CNP-induced meiotic arrest. COCs were cultured in medium containing 100 ng/mL CNP and different concentrations (0, 5, 10 and 50 nM) of estradiol. The proportion of oocytes with GV was evaluated after 6 and 8 hours of culture, respectively. B, Effect of estradiol on the expression of Npr2 mRNA. The Npr2 mRNA in COCs cultured in medium containing 100 ng/mL CNP and 10 nM estradiol was detected after 0, 2, 4, 6 and 8 hours of culture, respectively. CNP: C-type natriuretic peptide; Npr2: natriuretic peptide receptor 2; COCs: cumulus oocyte complexes; E2: estradiol.
Table 1.
Treatment with CNP during prematuration improved the maturation of goat oocytes matured in vitro.
Table 2.
Treatment with CNP during prematuration improved the developmental competence of goat oocytes matured in vitro.
Table 3.
Total cell number of blastocysts.