Fig 1.
Flow chart of the experimental design.
X indicates that these lines were not followed through to the F2 generation.
Table 1.
Mean seawater physiochemical conditions during the F1 adult and F2 larval and spat CO2 exposure experiments.
Fig 2.
The response of F1-control and F1-transgen Saccostrea glomerata adults to ambient and elevated CO2.
Extracellular pH (pHe) (a), standard metabolic rate (SMR) (b) after 5 w of exposure to the CO2 treatments (mean ± SE) (24°C, salinity 34.5 ppt).
Fig 3.
The response of F2-control and F2-transgen Saccostrea glomerata larvae to ambient and elevated CO2.
Percentage survival of larvae after 19 d (a), percentage eyed larvae after 19 d (b), percentage abnormality after 1 d (c), shell length after 19 d (d) of exposure to the CO2 treatments (mean ± SE) (24°C, salinity 34.5 ppt).
Fig 4.
Shell length of F2-control and F2-transgen Saccostrea glomerata larvae reared at ambient and elevated CO2.
Shell length measured at 2 d intervals from days 1–19 (mean ± SE) (24°C, salinity 34.5 ppt).
Fig 5.
The response of F2-control and F2-trangen Saccostrea glomerata spat to ambient (385 μatm) and elevated (856 μatm) CO2.
Shell growth (a), and heart rate (b) following 6 d of exposure to the CO2 treatments (mean ± SE). Spat were 14 d post settlement at the beginning of the exposure (24°C, salinity 34.5 ppt).